Magnetic hyperthermia therapy may be combined with controlled release of multiple chemotherapeutic agents to effectively treat cancerous tumors. This study describes the preparation and characteristics of gemcitabine- and paclitaxel-loaded magnetoliposomes to investigate the in vitro potential of this formulation in association with magnetic hyperthermia therapy to control drug delivery. Magnetic nanoparticles, gemcitabine, and paclitaxel were encapsulated into thermosensitive liposomes with efficiency of 84%, 57%, and 68%, respectively. The hydrodynamics and distribution of the magnetoliposome formulations were determined, showing ideal characteristics for accumulation in the tumor tissue: mean size less than 100 nm, polydispersity index < 0.2, and stability in aqueous suspension for at least 15 days. At physiological temperature, only 9% of the gemcitabine and 1% of the paclitaxel were released after 72 h, but the formulations exposed to an alternating magnetic field (AMF) dissipated energy sufficient to increase mean temperature to 43 °C in just 5 min and delivered 94% of the gemcitabine and 43% of the paclitaxel after 30 min. In vitro cytotoxicity and magnetic hyperthermia studies were then carried out using human primary breast cancer cells (MGSO-3) and MTT assay. The viability of cells exposed to the loaded magnetoliposomes and AMF for 30 min fell to 27%; the cells treated with the loaded magnetoliposomes but not exposed to AMF exhibited viability over 60%, while hyperthermia alone (with unloaded magnetoliposomes) reduced cell viability to 50%. This study consequently suggests that gemcitabine- and paclitaxel-loaded magnetoliposomes may present potential for combined treatments involving hyperthermia and controlled release of chemotherapeutic drugs.

磁热疗可以与多种化学治疗药物的控制释放相结合，以有效治疗癌性肿瘤。这项研究描述了吉西他滨和紫杉醇负载的磁脂质体的制备和特性，以研究该制剂与磁热疗法控制药物递送的体外潜力。将磁性纳米颗粒，吉西他滨和紫杉醇分别封装在热敏脂质体中，效率分别为84％，57％和68％。测定了磁脂质体制剂的流体力学和分布，显示出在肿瘤组织中积累的理想特征：平均尺寸小于100nm，多分散指数＜0.2，和在水悬浮液中至少15天的稳定性。在生理温度下 72小时后仅释放了9％的吉西他滨和1％的紫杉醇，但是暴露于交变磁场（AMF）的制剂耗散的能量足以在短短5分钟内将平均温度提高到43°C，并释放了94％的30分钟后吉西他滨和紫杉醇的43％。然后使用人原发性乳腺癌细胞（MGSO-3）和MTT分析进行了体外细胞毒性和磁热疗研究。暴露于负载的脂质体和AMF 30分钟的细胞活力降至27％；用负载的磁脂质体处理但未暴露于AMF的细胞显示出超过60％的活力，而单独的高温疗法（使用未负载的磁脂质体）将细胞活力降低到50％。