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Molecular evaluation of chronic restrain stress in mice model of non metastatic fibrosarcoma.
Journal of Molecular Histology ( IF 3.2 ) Pub Date : 2020-06-15 , DOI: 10.1007/s10735-020-09886-5
Gordana Smieško 1, 2 , Pavle Banović 3, 4 , Vera Gusman 1, 2 , Verica Simin 3 , Anca Maria Cimpean 5 , Dušan Lalošević 3, 6
Affiliation  

Chronic stress is regarded as a significant factor in the etiology of the many diseases. Numerous methods have been developed through which the effect of chronic stress is examined. The aim of this study is to demonstrate the new experimental model for analysis of immuno-suppression induced by chronic restraint stress, through challenge with conditionally tumorigenic cell line BHK-21/C13. 20 male NMRI mice were randomly divided into 2 groups—control and experimental. Each mouse was subcutaneously inoculated with BHK-21/C13 cells. Stress in the experimental group was induced for 20 days. After the experiment, tumor masses were removed, and analyzed using histology and immunohistochemistry techniques. We found a statistically significant difference (p = 0.034) in tumor expression and tumor volumes (p = 0.0061) between groups, as well as in immunopositivity on Ki67, cytochrome C and matrix metalloproteinase 9. Absence of immune infiltrate was noticed in experimental, and the presence of inflammatory infiltrate at tumor invasion front in control group.

中文翻译:

非转移性纤维肉瘤小鼠模型慢性束缚应激的分子评价。

慢性应激被认为是许多疾病病因学中的一个重要因素。已经开发了许多方法来检查慢性应激的影响。本研究的目的是通过条件性致瘤细胞系 BHK-21/C13 的挑战,展示用于分析慢性束缚应激诱导的免疫抑制的新实验模型。将 20 只雄性 NMRI 小鼠随机分为 2 组——对照组和实验组。每只小鼠皮下接种 BHK-21/C13 细胞。实验组应激诱导 20 天。实验后,切除肿瘤块,并使用组织学和免疫组织化学技术进行分析。我们发现组间肿瘤表达和肿瘤体积 (p = 0.0061) 存在统计学显着差异 (p = 0.034),
更新日期:2020-06-15
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