Purpose

The aims of the study were to identify microorganisms, including those in the VBNC state, inhabiting porous surfaces in oral surgery offices and to assess the biocidal effectiveness and impact of 300 ppm vaporised hydrogen peroxide (VHP) for 20 min on decontaminated materials.

Methods

From the surfaces of textured armrests of dental chairs, pinewood doors and window frames and cotton medical aprons, 30 swabs were taken with moistened sponges. The identification of isolated microorganisms was performed using molecular methods with MALDI-TOF MS, DNA Sanger sequencer and Illumina MiSeq. To evaluate the impact of VHP decontamination (independent variable) on the number of microorganisms (response variable) ANOVA and LSD tests were used. After application of 10 processes of VHP decontamination, changes in the properties of the materials were assessed using FTIR spectroscopy, SEM microscopy and XPS spectrometry.

Results

The concentration of microorganisms was 10¹–10⁴ CFU/100 cm² on the tested surfaces and 10² CFU/m³ in the air. Twenty species of bacteria, one yeast and 16 filamentous fungi were identified, with the predominance of Bacillus, Staphylococcus, Alternaria, Aspergillus and Penicillium. Moreover, Janthinobacterium, Acremonium, Aureobasidium, Coprinellus and Cosmospora in the VBNC state were metagenomically detected. VHP decontamination resulted in a reduction in the majority of tested microbial strains by a minimum of 3 log, and all tested mixed cultures inhabiting porous surfaces were above 98% and in the air, 100%. VHP decontamination did not affect the structural and morphological properties of cotton fibres, wood or stainless steel.

Conclusions

VHP decontamination at a concentration of 300 ppm for 20 min can be used for the holistic disinfection of air, surfaces and equipment in oral surgery offices.

中文翻译：

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使用汽化过氧化氢 (VHP) 对口腔外科诊所中微生物污染的非生物多孔表面进行净化

目的

该研究的目的是识别微生物，包括 VBNC 州的微生物，栖息在口腔外科办公室的多孔表面，并评估 300 ppm 汽化过氧化氢 (VHP) 20 分钟对净化材料的杀菌效果和影响。

方法

从牙科椅的纹理扶手、松木门窗框和棉质医用围裙的表面，用湿润的海绵采集了 30 个拭子。使用 MALDI-TOF MS、DNA Sanger 测序仪和 Illumina MiSeq 的分子方法对分离的微生物进行鉴定。为了评估 VHP 净化（自变量）对微生物数量（响应变量）的影响，使用了方差分析和 LSD 测试。在应用 10 道 VHP 去污工艺后，使用 FTIR 光谱、SEM 显微镜和 XPS 光谱评估材料性能的变化。

结果

被测表面的微生物浓度为 10 ¹ –10 ⁴  CFU/100 cm ^{2 ，空气中的微生物浓度为 10 2}  CFU/m ³。鉴定出细菌20种，酵母1种，丝状真菌16种，以芽孢杆菌属、葡萄球菌属、链格孢属、曲霉属和青霉菌属为主。此外，Janthinobacterium、Acremonium、Aureobasidium、Coprinellus和Cosmospora在 VBNC 状态下进行宏基因组检测。VHP 净化导致大多数测试微生物菌株减少至少 3 log，并且所有测试的居住在多孔表面的混合培养物均高于 98%，在空气中为 100%。VHP 净化不影响棉纤维、木材或不锈钢的结构和形态特性。

结论

300 ppm 浓度的 VHP 去污 20 分钟可用于口腔外科办公室的空气、表面和设备的整体消毒。