Brown rot disease caused by fungal species from the Monilinia genus has recently been observed as one of the most important limiting factors for yield and quality of peach fruits in Turkey. During June and July of 2018, field trips to different peach orchards were performed in six provinces located in four different geographical regions of Turkey. One hundred and twenty-nine isolates were obtained from the diseased fruits, and of those, 109 were identified as Monilinia fructicola, whereas 20 were M. laxa according to the species-specific molecular markers. Mating types of the isolates were detected by PCR assays using primers designed in this study. Each isolate represented only one of the mating type genes. Overall data sets presented 1:1 ratio of mating types for both species, indicating a possible sexual reproduction. No polymorphisms were detected in partial sequences of mating type genes. The isolates were in vitro tested for their pathogenicity using apple fruits as a host and aggressiveness were evaluated at different time points using lesion sizes. Monilinia laxa was found more aggressive than M. fructicola. Moreover, M. fructicola isolates displayed larger colony growth compared to M. laxa isolates. However, no significant correlation was detected between the colony growth rates and virulence. This study is the first to demonstrate brown rot pathogens of peach fruit in Turkey and identifies different aspects of the pathogens, which would be useful in containment of pathogen spread and comparison with other populations of these pathogens worldwide, and suggests a new set of mating type markers for these species.

最近，人们发现由莫尼利尼亚属的真菌引起的褐腐病是土耳其桃果实产量和品质的最重要限制因素之一。在2018年6月和7月期间，在土耳其四个不同地理区域的六个省进行了对不同桃园的实地考察。从患病的水果中获得了129个分离株，其中109个被鉴定为莫妮莉亚fructicola，而20个是M. laxa根据物种特异性的分子标记。使用本研究设计的引物通过PCR分析检测出分离株的交配类型。每个分离株仅代表一种交配型基因。总体数据集显示两种物种的交配类型比例为1：1，表明可能有性繁殖。在交配型基因的部分序列中未检测到多态性。使用苹果果实作为宿主，对分离株的致病性进行体外测试，并使用病灶大小在不同时间点评估其侵略性。发现Monilinia laxa比fructicola更具侵略性。此外，与松果油菜相比，毛果油菜菌株显示出更大的菌落生长。隔离株。但是，在菌落生长速率和毒力之间未发现显着相关性。这项研究是首次在土耳其展示桃子果实的褐色腐烂病原体，并确定了病原体的不同方面，这将有助于遏制病原体的传播并与世界各地这些病原体的其他种群进行比较，并提出一套新的交配类型这些物种的标记。