This research was conducted to examine the effect of altitude on primary metabolites (soluble solids, total sugars, invert sugars, individual invert sugars (glucose, fructose, sucrose), total acidity, pH, organic acids (citric acid, malic acid), vitamins (C, A, B1, B2, B3) and mineral contents (K, P, Ca, Mg, Na, Fe, Cu, Zn, Mo)) in black mulberry (Morus nigra) berry extracts. The experiment was performed at three locations: Location 1—(187 m altitude), Location 2—(271 m altitude) and Location 3—(493 m altitude). High performance liquid chromatography (HPLC) was employed for the identification of individual invert sugars, organic acids and vitamins (A, B1, B2, B3) in berry extracts. Vitamin C was evaluated by spectrophotometry, whereas the mineral contents were determined by flame atomic absorption spectrophotometry. The contents of primary metabolites, vitamins and minerals varied widely depending on altitude. The synthesis and accumulation of the tested parameters changed with increasing altitude. The highest levels of sugars were found in Location 3, whereas the values of acids, vitamin A and the minerals Na, Mo and Fe were higher in Location 1. The accumulation of macronutrients K and P, and vitamins C and B3 was most intensive in Location 2. Altitude had no effect on the contents of vitamin B1 and B2, and the micronutrients Cu and Zn. The results suggest that black mulberry fruits are rich in primary metabolites, vitamins and minerals, and that altitude has an important effect on the synthesis of the tested parameters in the fruits.

中文翻译：

---

取决于海拔高度的黑桑（浆果桑）浆果中主要代谢物，维生素和矿物质的测定

进行这项研究是为了研究海拔高度对主要代谢物（可溶性固体，总糖，转化糖，单个转化糖（葡萄糖，果糖，蔗糖），总酸度，pH，有机酸（柠檬酸，苹果酸），维生素的影响。黑桑（桑树）中的C，A，B1，B2，B3和矿物质含量（K，P，Ca，Mg，Na，Fe，Cu，Zn，Mo））浆果提取物。在三个位置进行了实验：位置1（海拔187 m），位置2（海拔271 m）和位置3（海拔493 m）。高效液相色谱（HPLC）用于鉴定浆果提取物中的各个转化糖，有机酸和维生素（A，B1，B2，B3）。通过分光光度法评估维生素C，而通过火焰原子吸收分光光度法测定矿物质含量。初级代谢产物，维生素和矿物质的含量随海拔高度变化很大。测试参数的合成和累积随高度的增加而变化。在位置3中发现糖的含量最高，而在位置1中酸，维生素A和矿物质Na，Mo和Fe的含量更高。在土壤中，大量营养素K和P的积累 维生素C和B3在位置2最密集。海拔高度对维生素B1和B2的含量以及微量营养元素Cu和Zn的含量没有影响。结果表明黑桑fruits果实富含初级代谢产物，维生素和矿物质，并且海拔高度对果实中测试参数的合成具有重要影响。