We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed base editing. By using paired sgRNAs, SWISS can produce insertions/deletions in addition to base editing. Rice mutants are generated using the SWISS system with efficiencies of cytosine conversion of 25.5%, adenine conversion of 16.4%, indels of 52.7%, and simultaneous triple mutations of 7.3%. The SWISS system provides a powerful tool for multi-functional genome editing in plants.

中文翻译：

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SWISS：使用 Cas9 切口酶和工程化 CRISPR RNA 支架在植物中进行多重正交基因组编辑

我们在此描述了由单一系统 (SWISS) 诱导的 CRISPR 同时和广泛编辑，其中在 crRNA 支架中设计的 RNA 适体将其与胞苷脱氨酶和腺苷脱氨酶融合的同源结合蛋白募集到 Cas9 切口酶目标位点，以产生多重碱基编辑。通过使用配对的 sgRNA，除了碱基编辑之外，SWISS 还可以产生插入/删除。水稻突变体是使用 SWISS 系统产生的，胞嘧啶转化率为 25.5%，腺嘌呤转化率为 16.4%，插入缺失率为 52.7%，同时三重突变率为 7.3%。SWISS 系统为植物中的多功能基因组编辑提供了强大的工具。