This study describes a microfluidic paper-based analytical device (μPAD) for separating plasma from whole blood and measuring glucose concentration. A two-dimensional μPAD was fabricated by wax printing, using chromatographic paper and was functionalized by the incorporation of chitosan into the plasma separation zone. Red blood cells were isolated from whole blood in the plasma separation zone consisting of a chitosan polymer structure and a wax barrier. The separated plasma, with buffer, was injected into the test zone to cause a color change. The colorimetric results were digitized with a scanner, and the concentration was calculated using a calibration curve. This μPAD, which separated the plasma from whole blood without a separation membrane, will be useful for constructing point-of-care testing devices that detect analytes in small sample volumes.

中文翻译：

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使用壳聚糖功能化的纸质分析设备的创新血浆分离方法。

这项研究描述了一种基于微流控纸的分析设​​备（μPAD），用于从全血中分离血浆并测量葡萄糖浓度。二维μPAD通过使用色谱纸的蜡印法制造，并通过将壳聚糖掺入血浆分离区而功能化。在由壳聚糖聚合物结构和蜡屏障组成的血浆分离区中，从全血中分离出红细胞。将分离的血浆和缓冲液注入测试区域，以引起颜色变化。用扫描仪将比色结果数字化，并使用校准曲线计算浓度。μPAD无需分离膜即可将血浆与全血分离，