LncRNA HOTTIP is a new lncRNA that is strictly linked to the susceptibility, growth, propagation, and prognosis of several human cancers together with colorectal cancer. lncRNA HOTTIP rs1859168 may confer colorectal cancer susceptibility through regulating its gene expression level. To elucidate its role in colorectal cancer risk, we genotyped rs1859168 A>C and measured serum HOTTIP expression level in colorectal cancer, adenomatous polyposis patients and controls by real-time polymerase chain reaction. The results displayed that rs1859168 A>C single-nucleotide polymorphism is a risk factor for colorectal cancer among adenomatous polyposis patients and controls, AC versus CC genotypes [adjusted odds ratio (OR) = 2.256, 95% confidence interval (CI) = 1.316–3.868, P = 0.003] when compared with controls and (adjusted OR = 9.521, 95% CI = 3.330–27.217, P < 0.0001) when compared with adenomatous polyposis. Serum HOTTIP was upregulated in the colorectal cancer group when compared with adenomatous polyposis or controls [median (interquartile range) = 3.64 (2.46–5.02) (P < 0.0001)]. A significant difference in serum HOTTIP was found to be associated with different rs1859168 genotypes. rs1859168 A>C and higher serum HOTTIP were significantly associated with distant metastasis, lymph nodes metastasis, and grade III of colorectal cancer. Both rs8159168 and high HOTTIP confer increased risk for colorectal cancer development.

中文翻译：

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rs1859168 / HOTTIP表达水平与结直肠癌和埃及人腺瘤性息肉病风险之间的关联。

LncRNA HOTTIP是一种新的lncRNA，与多种人类癌症以及大肠癌的易感性，生长，繁殖和预后密切相关。lncRNA HOTTIP rs1859168可通过调节其基因表达水平来赋予大肠癌易感性。为了阐明其在结直肠癌风险中的作用，我们对rs1859168 A> C进行了基因分型，并通过实时聚合酶链反应检测了结直肠癌，腺瘤性息肉病患者和对照中血清HOTTIP表达水平。结果显示，rs1859168 A> C单核苷酸多态性是腺瘤性息肉病患者和对照，AC和CC基因型[校正比值比（OR）= 2.256，95％置信区间（CI）= 1.316 – 3.868，P = 0.003]与对照组比较；（与校正过的相比，校正后的OR = 9.521，95％CI = 3.330–27.217，P  <0.0001）。与腺瘤性息肉病或对照组相比，大肠癌组血清HOTTIP上调[中位（四分位间距）= 3.64（2.46-5.02）（P  <0.0001）]。发现血清HOTTIP的显着差异与不同的rs1859168基因型有关。rs1859168 A> C和较高的血清HOTTIP与结直肠癌的远处转移，淋巴结转移和III级显着相关。rs8159168和高HOTTIP均增加了结直肠癌发展的风险。