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Spermine Regulates Water Balance Associated with Ca2+-Dependent Aquaporin (TrTIP2-1, TrTIP2-2 and TrPIP2-7) Expression in Plants under Water Stress.
Plant & Cell Physiology ( IF 4.9 ) Pub Date : 2020-06-16 , DOI: 10.1093/pcp/pcaa080
Zhou Li 1 , Jieru Hou 1 , Yan Zhang 1 , Weihang Zeng 1 , Bizhen Cheng 1 , Muhammad Jawad Hassan 1 , Youzhi Zhang 1 , Qi Pu 1 , Yan Peng 1
Affiliation  

Spermine (Spm) regulates water balance involved in water channel proteins, aquaporins (AQPs), in plants. An increase in endogenous Spm content via exogenous Spm application significantly improved cell membrane stability, photosynthesis, osmotic adjustment (OA) and water use efficiency (WUE) contributing to enhanced tolerance to water stress in white clover. Spm upregulated TrTIP2-1, TrTIP2-2 and TrPIP2-7 expressions and also increased the abundance of TIP2 and PIP2-7 proteins in white clover under water stress. Spm quickly activated intracellular Ca2+ signaling and Spm-induced TrTIP2-2 and TrPIP2-7 expressions could be blocked by Ca2+ channel blockers and the inhibitor of Ca2+-dependent protein kinase in leaves of white clover. TrSAMS in relation to Spm biosynthesis was first cloned from white clover and the TrSAMS was located in the nucleus. Transgenic Arabidopsis overexpressing the TrSAMS had significantly higher endogenous Spm content and improved cell membrane stability, photosynthesis, OA, WUE and transcript levels of AtSIP1-1, AtSIP1-2, AtTIP2-1, AtTIP2-2, AtPIP1-2, AtPIP2-1 and AtNIP2-1 than wild type in response to water stress. Current findings indicate that Spm regulates water balance via an enhancement in OA, WUE and water transport related to Ca2+-dependent AQP expression in plants under water stress.

中文翻译:

精胺调节水分胁迫下植物体内Ca2 +依赖性水通道蛋白(TrTIP2-1,TrTIP2-2和TrPIP2-7)的水平衡。

精胺(Spm)调节植物水通道蛋白,水通道蛋白(AQPs)中涉及的水分平衡。通过施用外源Spm来增加内源Spm含量可以显着改善细胞膜的稳定性,光合作用,渗透调节(OA)和水分利用效率(WUE),从而增强白三叶草对水分胁迫的耐受性。在水分胁迫下,Spm上调了TrTIP2-1TrTIP2-2TrPIP2-7的表达,并增加了白三叶草中TIP2和PIP2-7的含量。Spm迅速激活细胞内Ca 2+信号传导,而Spm诱导的TrTIP2-2TrPIP2-7表达可能被Ca 2+阻断通道阻滞剂和白三叶草叶片中Ca 2+依赖性蛋白激酶的抑制剂。与Spm生物合成相关的TrSAMS首先从白三叶草中克隆,TrSAMS位于细胞核中。过表达TrSAMS的转基因拟南芥具有显着更高的内源性Spm含量,并改善了AtSIP1-1AtSIP1-2AtTIP2-1AtTIP2-2AtPIP1-2AtPIP2-1AtSIP1-1AtSIP1-2AtTIP2-1的光合作用,OA,WUE和转录水平在NIP2-1比野生型更能应对水分胁迫。目前的发现表明,Spm通过增加OA,WUE和与水分胁迫下植物中Ca 2+依赖的AQP表达有关的水运输来调节水平衡。
更新日期:2020-06-16
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