ABSTRACT

Histo-blood group antigens (HBGAs) have been found to be important host susceptibility factors or receptors for human rotavirus (RVs) with genotype-specific host ranges, impacting the disease patterns, epidemiology, and strategy development against RV diseases in humans. However, how the glycan factors contribute to RV diversity and host ranges to different animal species remains unclear. In this study using recombinant VP8* proteins as probes to perform glycan array analyses of RVs, we observed a wide range of glycan-binding profiles, including those binding to sialic acid-containing glycans, among group A (RVA) and group C (RVC) RVs that mainly infect different animal species. A tri-saccharide glycan Galα1-3Galβ1-4Glc containing a terminal α-Gal was recognised by multiple RVA/RVC genotypes, providing valuable information on RV evolution under selection of the step-wisely synthesised HBGAs in many animals before they were introduced to humans to be human pathogens. Saliva binding studies of VP8* also revealed strain-specific host ranges or species barriers between humans and these animal RV genotypes, further improved our understanding on RV host ranges, disease burdens, epidemiology, and vaccine strategy against RVs.

摘要

已发现组织血型抗原（HBGA）是具有基因型特异性宿主范围的人轮状病毒（RVs）的重要宿主易感性因子或受体，影响疾病模式，流行病学以及针对人类RV疾病的策略制定。但是，聚糖因素如何促进RV多样性和宿主范围对不同动物物种的作用尚不清楚。在这项使用重组VP8 *蛋白作为探针进行RV的聚糖阵列分析的研究中，我们观察到A组（RVA）和C组（RVC）的各种聚糖结合谱，包括那些与含唾液酸的聚糖结合的谱。 ）主要感染不同动物物种的RV。含有末端α-Gal的三糖聚糖Galα1-3Galβ1-4Glc被多种RVA / RVC基因型识别，在选择逐步合成的HBGA之前，在许多动物中引入RV作为人类病原体之前，提供了有关RV进化的有价值的信息。VP8 *的唾液结合研究还揭示了人类与这些动物RV基因型之间特定菌株的宿主范围或物种障碍，进一步增进了我们对RV宿主范围，疾病负担，流行病学和针对RV的疫苗策略的了解。