Abstract

Artemisia herba-alba (Aha) is an aromatic and medicinal plant rich in health-promoting flavonoids compounds that play an important role as antioxidant. For better utilization of this resource, the ultrasound-assisted extraction (UAE) of flavonoids and antiradical activities of obtained extracts from Aha using response surface methodology was assessed. A four-factor five-level central composite rotatable design was employed to discuss these extracting parameters: ethanol concentration (X1), temperature (X2), extraction time (X3) and solvent to sample ratio (X4). The optimum extraction conditions for simultaneous maximization of total flavonoids (TF) and scavenging activity were solvent concentration of 50%, temperature of 55 °C, extraction time of 50 min and solvent to sample ratio of 90 mL/g. Under these conditions, the experimental yield of TF and DPPH-radical scavenging activity IC_50% were respectively 102.49 mg CE/g DM and 7 µg/mL. Additionally, good and moderate linear correlation was observed between antiradical activity and TF content (R² = 0.709) which indicated that these compounds are responsible for antioxidant activity of Aha. When compared to the most conventional extraction, antioxidant activity and amounts of major flavonoids are enhanced when using UAE with reduced extraction time.

摘要

茵陈蒿（Aha）是一种芳香和药用植物，富含促进健康的黄酮类化合物，这些化合物起着重要的抗氧化剂的作用。为了更好地利用该资源，使用响应面法评估了黄酮的超声辅助提取（UAE）和从Aha获得的提取物的抗自由基活性。采用四因素五级中心复合可旋转设计讨论了这些提取参数：乙醇浓度（X1），温度（X2），提取时间（X3）和溶剂与样品的比例（X4））。同时最大化总类黄酮（TF）和清除活性的最佳提取条件是溶剂浓度为50％，温度为55°C，提取时间为50分钟，溶剂与样品的比率为90 mL / g。在这些条件下，TF和DPPH自由基清除活性IC _50％的实验产率分别为102.49 mg CE / g DM和7 µg / mL。此外，在抗自由基活性和TF含量之间观察到良好和适度的线性相关性（R ² = 0.709），这表明这些化合物负责Aha的抗氧化活性。与最常规的提取方法相比，使用阿联酋减少提取时间可提高抗氧化活性和主要类黄酮的含量。