Aeromonas and Pseudomonas are important bacterial species involved in spoilage of refrigerated freshwater fish. In this study, 10 Aeromonas and seven Pseudomonas bacterial strains were isolated from spoiled grass carp and identified. Twelve of seventeen bacterial strains showed high potential of biofilm formation and 14 of 17 can produce extracellular protease. In order to explore the spoilage capacity of dual‐species, the sterile grass carp fillets were inoculated with mono‐ and dual‐species of Aeromonas salmonicida and Pseudomonas azotoformans strains. The results revealed significantly higher levels of the total viable count and total volatile basic nitrogen in dual‐species as compared to mono‐species from day 6. The higher contents of histamine, cadaverine and serious degradation in muscles tissue were also observed in dual‐species after 10 days of storage. Results of in vitro experiments showed that the co‐culture of A. salmonicida and P. azotoformans significantly increased the bacterial maximum growth rate, promoted the biofilm formation and improved the spoilage capacity of bacterial strains. This study has revealed that the co‐culture of Aeromonas and Pseudomonas bacterial strains accelerated spoilage process of grass carp and increased biofilm formation. It indicates that the mixed‐cultures of spoilage micro‐organisms pose a huge threat to food industry.

中文翻译：

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气单胞菌和假单胞菌细菌生物膜的共培养特性和冷藏草鱼的腐败潜力（Ctenopharyngodon idellu s）

气单胞菌和假单胞菌是涉及冷藏淡水鱼腐败的重要细菌种类。在这项研究中，从腐败的草鱼中分离并鉴定了 10 株气单胞菌和 7 株假单胞菌菌株。17 种细菌菌株中有 12 种显示出生物膜形成的高潜力，17 种中的 14 种可以产生细胞外蛋白酶。为了探索双品种的腐败能力，在无菌草鱼鱼片上接种杀鲑气单胞菌和偶氮假单胞菌的单种和双种菌株。结果显示，从第 6 天开始，双物种的总活菌数和总挥发性碱性氮水平显着高于单一物种。在储存 10 天后，在双物种中也观察到尸胺和肌肉组织的严重降解。体外实验结果表明，杀鲑鱼和偶氮假单胞菌共培养显着提高了细菌的最大生长速率，促进了生物膜的形成，提高了菌株的腐败能力。这项研究表明，气单胞菌和假单胞菌菌株的共培养加速了草鱼的腐败过程并增加了生物膜的形成。这表明腐败微生物的混合培养对食品工业构成了巨大威胁。促进生物膜形成并提高细菌菌株的腐败能力。这项研究表明，气单胞菌和假单胞菌菌株的共培养加速了草鱼的腐败过程并增加了生物膜的形成。这表明腐败微生物的混合培养对食品工业构成了巨大威胁。促进生物膜形成并提高细菌菌株的腐败能力。这项研究表明，气单胞菌和假单胞菌菌株的共培养加速了草鱼的腐败过程并增加了生物膜的形成。这表明腐败微生物的混合培养对食品工业构成了巨大威胁。