Protein adsorption is usually regarded as the main reason for filter fouling in sterile filtration of protein formulations. To achieve a better insight into this phenomenon, protein adsorption was studied during filtration of stabilized bovine serum albumin (BSA) and γ‐globulin formulations through 0.2‐µm microfilter membranes by inverse liquid chromatography (ILC). Adsorption processes can be studied with this method by measurement of breakthrough curves. The change of the concentration in the fluid phase is measured with high accuracy by an inline UV‐detector.

中文翻译：

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ILC无菌过滤蛋白质制剂过程中蛋白质吸附的研究

在蛋白质制剂的无菌过滤中，蛋白质吸附通常被认为是过滤器结垢的主要原因。为了更好地了解这种现象，在通过反相液相色谱（ILC）通过0.2 µm的微滤膜过滤稳定的牛血清白蛋白（BSA）和γ球蛋白制剂的过程中，研究了蛋白质吸附。可以通过测量穿透曲线来研究吸附过程。通过在线紫外线检测器可以高精度测量液相中的浓度变化。