Nitroxyl (HNO), a one‐electron reduction product of nitric oxide, demonstrates distinct biological and pharmacological activities. Here we designed a bioluminescent turn‐on probe, HNO‐8, that could be used to visualize HNO without the need for excitation light. HNO‐8 was prepared by caging 2‐hydroxyethyl luciferin with a triphenylphosphine unit, in which 2‐hydroxyethyl luciferin as a novel substrate of firefly luciferase was characterized by stronger and more sustained bioluminescent signals than the most popular substrates of d‐luciferin and 6′‐aminoluciferin. In vitro experiments showed that HNO‐8 could selectively respond to HNO generated from Angeli's salt(AS) in the range 1–50 μM, with a limit of detection of 0.196 μM. The probe was successfully applied for visualizing HNO in luciferase‐transfected Huh7 cancer cells. We envision that HNO‐8 could be used as a powerful bioluminescent sensor for researching HNO biological roles.

中文翻译：

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一种笼状 2-羟乙基荧光素，用于活细胞中硝酰基的生物发光成像。

Nitroxyl (HNO) 是一氧化氮的单电子还原产物，具有独特的生物和药理活性。在这里，我们设计了一种生物发光开启探针 HNO-8，可用于在不需要激发光的情况下可视化 HNO。HNO-8 是通过将 2-羟乙基萤光素与三苯基膦单元笼合制备的，其中 2-羟乙基萤光素作为萤火虫萤光素酶的新型底物，其生物发光信号比最流行的d-萤光素和 6'底物具有更强和更持久的生物发光信号。 -氨基荧光素。体外实验表明，HNO-8 在 1-50 μM 范围内对 Angeli's salt (AS) 产生的 HNO 有选择性响应，检测限为 0.196 μM。该探针成功应用于荧光素酶转染的 Huh7 癌细胞中 HNO 的可视化。我们设想 HNO-8 可用作研究 HNO 生物学作用的强大生物发光传感器。