Triple‐negative breast cancer (TNBC) is a highly heterogeneous disease. The aim of this study is to identify the diagnostic and poor prognostic signatures in TNBC by exploring the aberrant DNA methylation and gene expression. Differential expression and methylation analysis of the TNBC and paracancer samples from The Cancer Genome Atlas were performed. Gene set enrichment and protein–protein interaction (PPI) network analysis was used to explore the mechanisms of TNBC. Methylation‐gene expression correlation analysis was performed, and multivariate Cox analysis and receiver operating characteristics analysis were used to further screen the hub genes for TNBC. We identified 1,525 differentially expressed genes and 150 differentially methylated genes between TNBC and paracancer samples. About 96.64% of the methylation sites were located on the CpG island. A total of 17 Gene Ontology biological process terms and 18 signal pathways were significantly enriched. GNG4, GNG11, PENK, MAOA, and AOX1 were identified as the core genes of the PPI network. Methylation‐expression correlations revealed that ABCC9 (cg06951626), NKAPL (cg18675097, cg01031101, and cg17384889), and TMEM132C (cg03530754) showed promise as diagnostic and prognostic markers in TNBC. ABCC9 (cg06951626), NKAPL (cg18675097, cg01031101, and cg17384889), and TMEM132C (cg03530754) were potential diagnostic and prognostic markers in TNBC.

中文翻译：

---

ABCC9、NKAPL 和 TMEM132C 是三阴性乳腺癌的潜在诊断和预后标志物。

三阴性乳腺癌（TNBC）是一种高度异质性的疾病。本研究的目的是通过探索异常的 DNA 甲基化和基因表达来确定 TNBC 的诊断和不良预后特征。对来自癌症基因组图谱的 TNBC 和癌旁样本进行差异表达和甲基化分析。基因集富集和蛋白质 - 蛋白质相互作用（PPI）网络分析用于探索TNBC的机制。进行甲基化-基因表达相关分析，并使用多变量Cox分析和接受者操作特征分析进一步筛选TNBC的枢纽基因。我们在 TNBC 和癌旁样本之间鉴定了 1,525 个差异表达基因和 150 个差异甲基化基因。约 96。64% 的甲基化位点位于 CpG 岛上。共显着富集了17个基因本体生物过程术语和18条信号通路。GNG4、GNG11、PENK、MAOA和AOX1被确定为 PPI 网络的核心基因。甲基化-表达相关性显示ABCC9 (cg06951626)、NKAPL (cg18675097、cg01031101 和 cg17384889) 和TMEM132C (cg03530754) 在 TNBC 中显示出作为诊断和预后标志物的前景。ABCC9 (cg06951626)、NKAPL (cg18675097、cg01031101 和 cg17384889) 和TMEM132C (cg03530754) 是 TNBC 的潜在诊断和预后标志物。