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Nature-Inspired Adhesive Catecholamines for Highly Concentrated Colorimetric Signal in Spatial Biomarker Labeling.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-06-15 , DOI: 10.1002/adhm.202000540
Seunghwi Kim 1 , Seonki Hong 1
Affiliation  

Colorants have been utilized for precise biomarker detection in rapid and convenient colorimetric bioassays. However, the diffusion of colorants in solution often results in poor sensitivity, which is a major obstacle to the clinical translation of current colorants. To address this issue, in the current study, a unique colorant is developed that possesses adhesiveness for concentration near the target biomarker, avoiding diffusion. In nature, the synergistic interplay between catechol and amine functional groups is thought to be key for the unique mechanism of marine mussel adhesion. In addition, polymerized catecholamines are found in nature as biopigments, that is, in melanin. The dual role of catechol/catecholamine moieties in natural organics inspire to design novel colorimetric bioassays based on an adhesive colorant. Horseradish peroxidase (HRP) is used to initiate in situ polymerization of the catecholic precursors with amine‐containing additive molecules and simultaneously attach them near the HRP‐labeled biomarkers. This novel catecholamine‐based adhesive colorant provides an excellent quantitative (naked‐eye) visible signal and it also generates superb spatial information on the biomarkers on complex surfaces (e.g., cell membranes).

中文翻译:

在空间生物标记物标签中,以自然为灵感的胶粘剂儿茶酚胺,用于高度浓缩的比色信号。

着色剂已用于快速便捷的比色生物测定中的精确生物标志物检测。然而,着色剂在溶液中的扩散通常导致敏感性差,这是当前着色剂临床翻译的主要障碍。为了解决这个问题,在当前的研究中,开发了一种独特的着色剂,该着色剂对目标生物标志物附近的浓度具有粘附性,避免了扩散。在自然界中,邻苯二酚和胺官能团之间的协同相互作用被认为是海洋贻贝粘附的独特机制的关键。另外,在自然界中发现聚合的儿茶酚胺是生物色素,即在黑色素中。邻苯二酚/儿茶酚胺部分在天然有机物中的双重作用启发了人们设计基于粘合剂着色剂的新型比色生物测定法。辣根过氧化物酶(HRP)用于引发幼酚前体与含胺添加剂分子的原位聚合,并同时将其附着在HRP标记的生物标记附近。这种基于儿茶酚胺的新型粘合剂着色剂可提供出色的定量(裸眼)可见信号,并且还可在复杂表面(例如细胞膜)上的生物标志物上产生出色的空间信息。
更新日期:2020-08-19
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