Veterinary Parasitology ( IF 2.6 ) Pub Date : 2020-06-16 , DOI: 10.1016/j.vetpar.2020.109170 Paula Marchesini 1 , Alan Franco Barbosa 2 , Mirza Nalesso Gomes Sanches 3 , Rafael Moreira do Nascimento 4 , Francisca Leticia Vale 5 , Rodrigo Luiz Fabri 6 , Ralph Maturano 4 , Mário Geraldo de Carvalho 3 , Caio Monteiro 7
The present study was carried out to evaluate and compare the acaricidal activity of different fractions of Acmella oleracea methanolic extract, containing 0.0 % (F1), 24.5 % (F2), 48.0 % (F3) and 100 % (F4) of spilanthol, on unfed larvae and engorged females from the same Rhipicephalus microplus population. To obtain these fractions, the crude extract was subjected to different extraction procedures using increasingly polarized solvents to isolate the spilanthol compound. The Larval Packet Test was used to evaluate acaricidal activity in unfed larvae at concentrations ranging from 0.2 to 25.0 mg/mL, while for engorged females, the Adult Immersion Test was performed at concentrations from 3.1 to 25.0 mg/mL. The F1 fraction showed no activity on unfed larvae, while a control percentage of 44.6 % was observed at a concentration of 25.0 mg/mL for engorged females. For unfed larvae, the F2 fraction resulted in 95.7 % mortality at a concentration of 1.6 mg/mL, with a control percentage of 92.7 % for engorged females at a concentration of 12.5 mg/mL. Fractions F3 and F4 had similar activity against unfed larvae, with mortality >84.0 % from the concentration of 0.8 mg/mL. This similarity between the fractions was also observed for engorged females from a concentration of 12.5 mg/mL, resulting a control percentage >94.0 %. These results demonstrate that the presence of spilanthol is an important factor for the acaricidal activity of A. oleracea extract. Fraction extracts with 24.5, 48 and 100 % of spilanthol have similar acaricidal activity on R. microplus.
中文翻译:
沙棘(Asterella oleracea)(菊科)提取物对微小头R(Rhipicephalus microplus)的杀螨活性:二十碳五烯醇的影响是什么?
进行本研究以评估和比较不同等级的油茶无水甲醇提取物的杀螨活性,该提取物含有0.0%(F1),24.5%(F2),48.0%(F3)和100%(F4)的七氢薄荷醇。相同的Rhipicephalus microplus的未喂食幼虫和饱食的雌性人口。为了获得这些馏分,使用逐渐极化的溶剂对粗提物进行不同的提取程序,以分离出香波醇化合物。幼虫数据包试验用于评估未喂食幼虫在0.2至25.0 mg / mL范围内的杀螨活性,而对于饱腹的雌性,成年浸没试验在3.1至25.0 mg / mL的浓度下进行。F1分数对未喂食的幼虫没有活性,而对于饱腹的雌性,当浓度为25.0 mg / mL时,观察到的控制百分比为44.6%。对于未喂食的幼虫,F2分数在1.6 mg / mL的浓度下可导致95.7%的死亡率,而在12.5 mg / mL的浓度下对饱腹雌性的控制率为92.7%。馏分F3和F4对未喂食幼虫具有相似的活性,死亡率> 84。从0.8 mg / mL的浓度为0%。对于浓度为12.5 mg / mL的饱足雌性,也观察到各部分之间的相似性,从而导致对照百分比> 94.0%。这些结果表明,五香酚的存在是其杀螨活性的重要因素。A. oleracea提取物。含有24.5%,48%和100%的香薄荷醇的级分提取物具有对R. microplus相似的杀螨活性。