Abstract In this work, we synthesized fluorescent chicken egg white mediated platinum nanoclusters (CEW-Pt NCs) via one-step synthetic approach using chicken egg white as template as well as stabilizing agent. The-as synthesized fluorescent CEW-Pt NCs gives blue fluorescence at 365 nm of UV lamp. The fluorescent CEW-Pt NCs exhibit the emission at 406 nm when excited at 330 nm. Taking CEW-Pt NCs as a model nanoprobe, the fluorescence “turn-off” mechanism is developed for assaying of carbidopa based on the linear quenching of emission peak intensity at 406 nm. The carbidopa acts as a quencher, resulting to exhibit a linear response in the range of 5.0–35 μM with the detection limit of 1.71 μM. The fluorescence quenching of CEW-Pt NCs exhibits selectivity toward carbidopa over other similar drugs and chemical (inorganic and biomolecules). This method is shown to be a miniaturized analytical platform for assaying of carbidopa in its pharmaceutical samples and biofluids, which could open up a new and straightforward platform for assaying of carbidopa in real samples.

中文翻译：

---

鸡蛋清介导合成铂纳米簇用于选择性检测卡比多巴

摘要 在这项工作中，我们以鸡蛋清为模板和稳定剂，通过一步合成方法合成了荧光鸡蛋清介导的铂纳米簇（CEW-Pt NCs）。合成的荧光 CEW-Pt NCs 在 365 nm 的紫外灯下发出蓝色荧光。当在 330 nm 激发时，荧光 CEW-Pt NCs 在 406 nm 处发射。以 CEW-Pt NCs 作为模型纳米探针，基于 406 nm 发射峰强度的线性猝灭，开发了用于检测卡比多巴的荧光“关闭”机制。卡比多巴充当猝灭剂，导致在 5.0–35 μM 范围内表现出线性响应，检测限为 1.71 μM。CEW-Pt NCs 的荧光猝灭表现出对卡比多巴的选择性，而不是其他类似的药物和化学物质（无机和生物分子）。