Integration of disease diagnosis and therapy in vivo by nanotechnology is a challenge in the design of multifunctional nanocarriers. Herein, we report an intelligent and degradable nanoreactor, an assembly of the 4-mercaptobenzonitrile-decorated silver nanoparticles (AgNPs@MBN) and the glucose oxidase (GOx)-loaded metal-organic-framework (ZIF-8@GOx), which can be activated by tumor microenvironment to start the catalytic cascade-enhanced chemo-starvation synergistic therapy and simultaneous self-sense of cellular glucose level. Under the mild acidic microenvironment of tumor, the nanoreactor will collapse to release GOx that triggers a catalytic cascade reaction in vivo, depleting glucose, etching AgNPs@MBN, and producing toxic H₂O₂, Ag⁺, and Zn²⁺ ions, all of which work together to inhibit tumor growth. The AgNPs@MBN as SERS nanoprobe reads out glucose concentration noninvasively in tumor to achieve instant feedback of therapeutic progression. This work proposes a promising example of using enzyme-encapsulated biomineralized MOFs as an effective anticarcinogen for clinical applications.

通过纳米技术在体内整合疾病诊断和治疗是多功能纳米载体设计中的一个挑战。在这里，我们报告了一种智能且可降解的纳米反应器，它是由4-巯基苯甲腈修饰的银纳米颗粒（AgNPs @ MBN）和葡萄糖氧化酶（GOx）负载的金属有机框架（ZIF-8 @ GOx）组成的组件。被肿瘤微环境激活后，开始催化级联增强的化学饥饿协同治疗，并同时自我感知细胞葡萄糖水平。在肿瘤的轻度酸性微环境下，纳米反应器将坍塌以释放GOx，从而触发体内的催化级联反应，消耗葡萄糖，蚀刻AgNPs @ MBN并产生有毒的H ₂ O _2。，Ag ⁺和Zn ²⁺离子，它们全部共同作用以抑制肿瘤的生长。作为SERS纳米探针的AgNPs @ MBN可无创地读出肿瘤中的葡萄糖浓度，以实现治疗进展的即时反馈。这项工作提出了一个有希望的例子，将酶囊化的生物矿化的MOF用作临床应用的有效抗癌剂。