Linden tea has been used in Serbian folk medicine for centuries to induce sweating for colds, relieve throat irritation and cough, reduce blood pressure, as well as a diuretic, spasmolytic, and as a sedative. The main aim of this study was to determine the comprehensive metabolite profile, free radical scavenging activity, and antimicrobial activities against 23 human and plant pathogens of both commercial and field-collected linden tea samples from Serbia. Developed high-performance thin-layer chromatography (HPTLC) method allowed identification of eight major metabolites in investigated samples, while forty-six metabolites were tentatively identified using high resolution mass spectrometry (HRMS): 14 phenolic acids and their derivatives, 15 glycosides, 9 flavonoids, and 8 procyanidins. Both commercial and field-collected linden tea samples showed similar total phenolic content and radical scavenging activity. Seven compounds such as p-hydroxybenzoic acid 4-O-hexoside, caffeic acid cinnamyl ester, pinocembrin, galangin, luteolin 7-O-glucuronide (and its isomer), isorhamnetin hexosyl hexoside were found in Tilia samples for the first time. Radical reagent 2,2-Diphenyl-1-picrylhydrazyl· (DPPH·) was used in DPPH-HPTLC assay which identified chlorogenic acid, caffeic acid, astragalin, quercitrin, tiliroside, and rutin as compounds that exhibit radical scavenging activity. Tilia samples showed inhibitory effect overwhelming only on Gram-positive bacteria, especially on Bacillus subtilis with the lowest MIC values observed, as well as towards Staphylococcus aureus and oral cavity isolates of Streptococcus pyogenes and Streptococcus mutans. Vaginal isolate of Candida glabrata showed more susceptibility than Candida albicans isolate. Generally, extract from Tilia cordata Miller (L4) showed the highest antimicrobial activity against the most of the tested pathogens, among all field-collected Tilia samples.

菩提茶已在塞尔维亚民间医学中使用了几个世纪，它可以诱发出汗，感冒，缓解咽喉刺激和咳嗽，降低血压，以及利尿剂，解痉剂和镇静剂。这项研究的主要目的是确定对来自塞尔维亚的商业和现场收集的树茶样品的23种人类和植物病原体的综合代谢物谱，自由基清除活性和抗菌活性。开发的高效薄层色谱法（HPTLC）可以鉴定所研究样品中的8种主要代谢物，而使用高分辨率质谱（HRMS）初步鉴定了46种代谢物：14种酚酸及其衍生物，15种糖苷，9种类黄酮和8种原花青素。商业和现场收集的树茶样品均显示出相似的总酚含量和自由基清除活性。七种化合物，例如在T树属样品中首次发现对羟基苯甲酸4- O-己糖苷，咖啡酸肉桂酸酯，松皮精，高良姜精，木犀草素7- O-葡糖醛酸苷（及其异构体），异鼠李素六糖基己糖苷。在DPPH-HPTLC分析中使用了自由基试剂2,2-二苯基-1-吡啶并肼基（DPPH ·），该试剂鉴定了绿原酸，咖啡酸，黄芪素，槲皮苷，tiliroside和芦丁为具有自由基清除活性的化合物。ilia树皮样品显示出抑制作用，仅对革兰氏阳性细菌具有抑制作用，尤其是对MIC最低的枯草芽孢杆菌以及金黄色葡萄球菌具有抑制作用化脓性链球菌和变形链球菌的口腔分离株。与白色念珠菌分离株相比，光滑念珠菌的阴道分离株表现出更高的敏感性。通常，在所有田间采集的T虫样品中，T虫米勒（L4）的提取物对大多数被测病原体表现出最高的抗菌活性。