Dairy products have been implicated in foodborne infections caused by different bacterial pathogens. Among them, Listeria monocytogenes is of particular concern due to its ubiquity, resistance to sanitation processes and high mortality rates resulting from infection. These issues make the development of novel methods for the rapid detection of this bacterium of high interest. The evaluation of a novel multiplex real-time Recombinase Polymerase Amplification method including an internal amplification control is reported in the present work. The method performance was compared to that of the European reference method (ISO 11290-1) for the detection of the species in samples from 40 commercial products, including 14 UHT milk samples, 16 hard cheese samples, 6 infant dairy preparation samples and 4 fresh cheese samples. A limit of detection below 10 cfu/25 g or mL sample was achieved, and values higher than 90% were obtained for relative sensitivity, specificity, accuracy, positive and negative predictive values and the index (kappa) of concordance. Analysis was achieved within one working day, compared to the six days required using the ISO method. Moreover, slight modification of the ISO 11290-1 method to include secondary enrichment in half Fraser broth resulted in the confirmation of all positive samples.

乳制品与由不同细菌病原体引起的食源性感染有关。其中，单核细胞增生李斯特菌由于其无处不在，对卫生过程的抵抗力以及感染导致的高死亡率，这一点尤其令人关注。这些问题使得快速检测这种高度关注的细菌的新方法的发展。在本工作中报道了对包括内部扩增对照的新型多重实时重组酶聚合酶扩增方法的评估。将方法性能与欧洲参考方法（ISO 11290-1）的方法性能进行了比较，以检测40种商业产品样品中的物种，包括14种UHT牛奶样品，16种硬干酪样品，6种婴儿乳制品样品和4种新鲜奶酪样品。样品的检出限低于10 cfu / 25 g或mL，相对灵敏度，特异性，准确性，正预测值和负预测值以及一致性指数（kappa）。与使用ISO方法所需的六天相比，在一个工作日内即可完成分析。此外，对ISO 11290-1方法进行的轻微修改，包括将一半的Fraser肉汤进行二次富集，可以确认所有阳性样品。