Platelet-derived growth factor (PDGF) is associated with clinical proliferative vitreoretinopathy (PVR), which is characterized by formation of sub- or epi-retinalmembranes that consist of cells including retinal pigment epithelial （ RPE ） cells and extracellular matrix. RPE cells play an important role in PVR pathogenesis. Previous findings indicated that PDGF receptor (PDGFR)α was essential in experimental PVR induced by fibroblasts. In RPE cells derived from epiretinal membranes from patients with PVR (RPEMs) ， Akt was activated by PDGF-B but not PDGF-A, which suggested that PDGFRβ was the predominant PDGFR isoform expressed in RPEMs. Indeed, CRISPR/Cas9-mediated depletion of PDGFRβ attenuated patient vitreous-induced Akt activation and cellular responses intrinsic to PVR including cell proliferation, migration, and contraction. We conclude that PDGFRβ appears to be the relevant PDGFR isoform in RPEMs.

血小板衍生生长因子（PDGF）与临床增生性玻璃体视网膜病变（PVR）相关，其特征是形成视网膜下膜或视网膜上膜，该膜由视网膜色素上皮（RPE）细胞和细胞外基质组成。RPE细胞在PVR发病机理中起重要作用。先前的发现表明，PDGF受体（PDGFR）α在成纤维细胞诱导的实验性PVR中至关重要。在PVR患者（RPEM）的视网膜前膜来源的RPE细胞中，Akt被PDGF-B激活，但未被PDGF-A激活，这表明PDGFRβ是RPEM中表达的主要PDGFR亚型。确实，CRISPR / Cas9介导的PDGFRβ耗竭减弱了患者玻璃体诱导的Akt激活和PVR固有的细胞反应，包括细胞增殖，迁移和收缩。