Marine biofouling is perplexing the development of marine industry, and the traditional antifouling methods are restricted by the requirements of marine environmental friendliness. Marine bacteria attachment is the initial stage of marine fouling and it can be effectively reduced by reducing bacterial attachment. In this study, two modification methods were reported to synthesize antibacterial surfaces based on the different order of Magainin Ⅱ (MAG Ⅱ) modification. The preparation of SS-DA-M was generated by modifying the 304 stainless steel (304 SS) surface with dopamine firstly and then grafting the MAG Ⅱ onto the dopamine modified surface; SS-M-DA was obtained by modifying 304 SS surface using MAG Ⅱ derivative which synthesized by MAG Ⅱ and dopamine under weak acid condition. XPS, contact angle, film thickness and surface topography analysis showed that both methods grafted MAG Ⅱ onto the 304 SS surface successfully, changing the morphology and wettability of the substrates. Antibacterial results demonstrated that the two modified surfaces possessed strong resistance against V. natriegens, and the antibacterial efficiency of SS-DA-M and SS-M-DA reached 98.07 % and 99.79 %, respectively. Robustness results illustrated that the modified surface could keep strong antibacterial capacity in seawater for a long time. The phy-chemical properties and antibacterial properties of SS-M-DA surface were superior to SS-DA-M surface because more MAG Ⅱ were grafted onto 304 SS surface and the distribution was more uniform than the SS-DA-M surface. The investigation may offer a new and promising strategy to tackle surface fouling of hull, aquaculture cage and other marine facilities.

海洋生物污损困扰着海洋工业的发展，传统的防污方法受到海洋环境友好性要求的限制。海洋细菌附着是海洋污染的初始阶段，可以通过减少细菌附着来有效减少。在这项研究中，据报导了两种修饰方法可以根据不同顺序对MagaininⅡ（MAGⅡ）进行修饰来合成抗菌表面。首先用多巴胺修饰304不锈钢（304 SS）表面，然后将MAGⅡ接枝到多巴胺修饰的表面上，制备SS-DA-M。通过在弱酸条件下用MAGⅡ与多巴胺合成的MAGⅡ衍生物对304 SS表面进行改性，得到SS-M-DA。XPS，接触角，膜厚和表面形貌分析表明，两种方法均成功地将MAGⅡ接枝到304 SS表面，改变了基材的形貌和润湿性。抗菌结果表明，两个修饰的表面具有很强的抵抗力V. natriegens，SS-DA-M和SS-M-DA的抗菌效率分别达到98.07％和99.79％。鲁棒性结果表明，改性后的表面可以长时间保持强的抗菌能力。SS-M-DA表面的理化性质和抗菌性能优于SS-DA-M表面，这是因为更多的MAGⅡ被接枝到304 SS表面上，并且分布比SS-DA-M表面更均匀。该调查可能会为解决船体，水产养殖网箱和其他海洋设施的表面污垢提供一种新的有希望的战略。