AgNPs were immobilized on titania nanotubes (TNT) by chelation of polydopamine (PD) to generate a TNT/PD/AgNPs (TPAS) via a simple dipping method. The inflammatory regulation of the TPAS coating were investigated. To gain a deep insight into the transformation of AgNPs in macrophages, a cation exchange reaction was introduced for speciation analysis of AgNPs and Ag⁺ by inductively coupled plasma-mass spectrometry. Owing to the magic signal amplification strategy, the trace AgNPs and Ag⁺ in release media and even in macrophages were easily detected. In simulated inflammatory microenvironment, M1 macrophages promoted the cell-responsive release of Ag⁺ from TPAS at 3 d, which dampened inflammation. Then, macrophages reduced Ag⁺ by intracellular metabolites, leading to the formation of new AgNPs in cells. This study give a new sight for discovering the inflammatory regulation mechanism of silver containing biomaterials.

通过多巴胺（PD）的螯合将AgNPs固定在二氧化钛纳米管（TNT）上，通过简单的浸渍方法生成TNT / PD / AgNPs（TPAS）。研究了TPAS涂层的炎症调节。为了深入了解巨噬细胞中AgNP的转化，引入了阳离子交换反应，用于通过电感耦合等离子体质谱法对AgNP和Ag ⁺进行形态分析。由于采用了神奇的信号放大策略，可以轻松检测释放介质甚至巨噬细胞中的痕量AgNPs和Ag ⁺。在模拟的炎症微环境中，M1巨噬细胞在3 d时促进了TPAS中的细胞响应性释放Ag ⁺，从而减轻了炎症。然后，巨噬细胞还原了Ag ⁺通过细胞内代谢产物，导致在细胞中形成新的AgNP。这项研究为发现含银生物材料的炎症调节机制提供了新的视野。