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Identification of QTL and genes for pod number in soybean by linkage analysis and genome-wide association studies
Molecular Breeding ( IF 3.1 ) Pub Date : 2020-06-15 , DOI: 10.1007/s11032-020-01140-w
Jie Song , Xu Sun , Kaixin Zhang , Shulin Liu , Jiajing Wang , Chang Yang , Sitong Jiang , Mahfishan Siyal , Xiyu Li , Zhongying Qi , Yue Wang , Xiaocui Tian , Yanlong Fang , Zhixi Tian , Wen-Xia Li , Hailong Ning

Pod number is an important component of yield in soybean (Glycine max (L.) Merr.), and pods are unevenly distributed in the upper, middle and lower parts of the plant. Here, linkage analysis combined with genome-wide association study (GWAS) was used to identify 20 pod number-related traits in four-way recombinant inbred lines (FW-RILs) derived from crosses between the four soybean varieties (Kenfeng 14 × Kenfeng 15) × (Heinong 48 × Kenfeng 19). The results show that pod number-related traits are highly influenced by genetic factors, while the environment plays only a minor role. A total of 602 QTLs were identified, of which 52 were detected in multiple environments (over two environments). In addition, GWAS detected 26 common QTNs (identified by multiple methods or environments) in the QTL intervals. Based on gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, 11 potential candidate genes were identified that likely involved the growth and development of soybean pods. These findings will help elucidate the genetic basis of pod number traits.



中文翻译:

通过连锁分析和全基因组关联研究鉴定大豆的QTL和荚果基因

荚数是在大豆(产率的重要组成部分大豆(L.)Merr。),并且豆荚不均匀地分布在植物的上部,中部和下部。在这里,连锁分析与全基因组关联研究(GWAS)相结合,被用于鉴定来自四个大豆品种(Kenfeng 14×Kenfeng 15)杂交的四向重组自交系(FW-RILs)的20个与荚果数量相关的性状。 )×(Heinong 48×Kenfeng 19)。结果表明,与豆荚数量相关的性状受遗传因素的影响很大,而环境仅扮演很小的角色。总共确定了602个QTL,其中在多个环境(两个环境中)中检测到52个。此外,GWAS在QTL间隔中检测到26个常见QTN(通过多种方法或环境标识)。根据基因本体论(GO)富集分析和《京都基因与基因组百科全书》(KEGG)分析,确定了11个潜在的候选基因,这些基因可能与大豆荚的生长发育有关。这些发现将有助于阐明荚果数量性状的遗传基础。

更新日期:2020-06-15
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