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Inter-particle biomolecular reactivity tuned by surface crowders.
Nanoscale ( IF 6.7 ) Pub Date : 2020-06-15 , DOI: 10.1039/d0nr03125a
M R W Scheepers 1 , S R R Haenen , J M Coers , L J van IJzendoorn , M W J Prins
Affiliation  

The rate at which colloidal particles can form biomolecular bonds controls the kinetics of applications such as particle-based biosensing, targeted drug delivery and directed colloidal assembly. Here we study how the reactivity of the particle surface depends on its molecular composition, quantified by the inter-particle rate of aggregation in an optomagnetic cluster experiment. Particles were functionalized with DNA or with proteins for specific binding, and with polyethylene glycol as a passive surface crowder. The data show that the inter-particle binding kinetics are dominated by specific interactions, which surprisingly can be tuned by the passive crowder molecules for both the DNA and the protein system. The experimental results are interpreted using model simulations, which show that the crowder-induced decrease of the particle surface reactivity can be described as a reduced reactivity of the specific binder molecules on the particle surface.

中文翻译:

通过表面拥挤物调节颗粒间的生物分子反应性。

胶体颗粒形成生物分子键的速率控制着应用的动力学,例如基于颗粒的生物传感,靶向药物递送和定向胶体组装。在这里,我们研究粒子表面的反应性如何取决于其分子组成,该分子组成由光磁簇实验中的粒子间聚集速率来量化。用DNA或蛋白质对颗粒进行功能化以实现特异性结合,并使用聚乙二醇作为被动表面拥挤剂。数据表明,颗粒间的结合动力学受特定相互作用的控制,这令人惊讶地可以通过被动拥挤分子对DNA和蛋白质系统进行调节。实验结果通过模型仿真来解释,
更新日期:2020-07-16
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