Coat proteins have a central role in vesicular transport by binding to cargoes for their sorting into intracellular pathways. Cargo recognition is mediated by components of the coat complex known as adaptor proteins^1,2,3. We previously showed that Arf-GAP with coil-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) functions as an adaptor for a clathrin coat complex that has a function in endocytic recycling^4,5,6. Here, we show that the protein kinase Akt acts as a co-adaptor in this complex, and is needed in conjunction with ACAP1 to bind to cargo proteins to promote their recycling. In addition to advancing the understanding of endocytic recycling, we uncover a fundamentally different function in which a kinase acts, as Akt in this case is an effector rather than a regulator in a cellular event.

外壳蛋白通过与货物结合以分类进入细胞内途径，在水泡运输中起核心作用。货物识别是通过被称为衔接蛋白^1,2,3的外壳复合物的成分介导的。我们先前显示带有线圈，ANK重复序列和含PH结构域蛋白1（ACAP1）的Arf-GAP可作为网格蛋白外壳复合物的衔接子，该复合物在胞吞循环中具有功能^4,5,6。在这里，我们证明了蛋白激酶Akt在这种复合物中起着共同适配器的作用，并且需要与ACAP1结合以结合货物蛋白以促进其再循环。除了增进对内吞再循环的理解外，我们还揭示了激酶起作用的根本不同功能，因为在这种情况下，Akt在细胞事件中是效应子而不是调节子。