Background: Allergic rhinitis is the most prevalent atopic disorder worldwide. Inflammation is believed to participate in allergic rhinitis. Previous studies indicate that hypoxia-inducible factor (HIF) promotes the development of allergic rhinitis, and dendritic cells are also involved in allergic rhinitis. Methods:We explored the consequences of HIF1α deficiency in dendritic cells on allergic rhinitis. Allergic rhinitis in mice was induced by ovalbumin (OVA). The levels of IgE, leukotriene C4 (LTC4), eosinophil cationic protein (ECP), prostaglandin D2 (PGD2), IFN-γ, IL-2, IL-4, IL-5, IL-10, and IL-13 in serum or nasal lavage fluid (NLF) were detected by ELISA. Inflammatory cells in NLF were counted by hemocytometer. The protein levels of p-ERK1/2, p-p38, p-JNK2, SIRT1, p-IκBα, and p65 were determined by Western blot. Results:HIF1α deficiency in dendritic cells (HIF1α^CD11c–/–) decreased sneezing and nasal rubbing, the production of OVA-specific IgE, LTC4, and ECP in serum and NLF, and the numbers of leukocytes, eosinophils, lymphocytes, and neutrophils in NLF. Th1 cytokines increased, while Th2 cytokines decreased in HIF1a^CD11c–/– mice. SIRT1/NF-κB signaling was inhibited in HIF1α^CD11c–/– mice, while SIRT1 inhibitor administration in HIF1α^CD11c–/– mice attenuated the symptoms and inflammatory indicators of allergic rhinitis. Conclusion:HIF1α deficiency in dendritic cells attenuates symptoms and inflammatory indicators of allergic rhinitis in a SIRT1-dependent manner.
Int Arch Allergy Immunol

中文翻译：

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树突状细胞中的HIF1α缺乏以SIRT1依赖性方式减轻变应性鼻炎的症状和炎症指标。

背景：过敏性鼻炎是全球最普遍的特应性疾病。据信炎症参与过敏性鼻炎。先前的研究表明，缺氧诱导因子（HIF）促进变应性鼻炎的发展，并且树突状细胞也参与变应性鼻炎。方法：我们探讨了树突状细胞中HIF1α缺乏对过敏性鼻炎的后果。卵白蛋白（OVA）诱发小鼠过敏性鼻炎。血清中IgE，白三烯C4（LTC4），嗜酸性粒细胞阳离子蛋白（ECP），前列腺素D2（PGD2），IFN-γ，IL-2，IL-4，IL-5，IL-10和IL-13的水平ELISA检测鼻腔灌洗液或鼻灌洗液（NLF）。用血细胞计数器计数NLF中的炎性细胞。通过蛋白质印迹法测定p-ERK1 / 2，p-p38，p-JNK2，SIRT1，p-IκBα和p65的蛋白水平。结果：树突状细胞中的HIF1α缺乏（HIF1αCD11c ^{– / –}）减少打喷嚏和鼻擦，血清和NLF中OVA特异性IgE，LTC4和ECP的产生以及NLF中白细胞，嗜酸性粒细胞，淋巴细胞和中性粒细胞的数量减少。在HIF1a ^{CD11c – / –}小鼠中，Th1细胞因子增加，而Th2细胞因子减少。SIRT1 / NF-κB信号传导在HIF1α抑制^{的CD11c - / -}小鼠中，而在HIF1αSIRT1抑制剂给药^{的CD11c - / -}小鼠中减毒症状和过敏性鼻炎的炎症指标。结论：树突状细胞中HIF1α缺乏以SIRT1依赖性方式减轻变应性鼻炎的症状和炎症指标。
Int Arch过敏免疫