In this study, an efficient methodology, allowing the controlled co‐immobilization of two complementary biomolecules, is reported for the production of multifunctional antibacterial surfaces. To promote long‐lasting covalent immobilization, metallic surfaces are first coated with a quinone‐bearing poly(methacrylate)‐based thin film by combining an atmospheric pressure liquid‐assisted plasma polymerization and a controlled sodium periodate‐induced catechol oxidation steps. The influence of the oxidation step on the film morphology and chemistry is investigated using an analytical multitool approach involving atomic force microscopy, ultraviolet, infrared, and X‐ray photoelectron spectroscopy techniques. Quartz crystal microbalance with dissipation monitoring (QCM‐D) analyses allow the rapid determination of the optimal biomolecule immobilization conditions in terms of kinetics of grafting and biomolecule solution concentrations. In vitro functional assays combined with QCM‐D analyses demonstrate promising, dual biologically active coated surfaces.

中文翻译：

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可控制的生物分子在含醌的等离子聚合物薄膜上的共固定化，用于多功能生物材料表面

在这项研究中，据报道，一种有效的方法可以控制两种互补生物分子的共固定化，以生产多功能抗菌表面。为了促进持久的共价固定化，首先通过结合大气压液体辅助等离子体聚合和可控的高碘酸钠诱导的邻苯二酚氧化步骤，在金属表面涂上含醌的聚（甲基丙烯酸酯）基薄膜。使用包括原子力显微镜，紫外线，红外线和X射线光电子能谱技术在内的多种分析工具，研究了氧化步骤对薄膜形态和化学的影响。带有耗散监测（QCM-D）分析的石英晶体微天平可以根据接枝动力学和生物分子溶液浓度快速确定最佳生物分子固定条件。体外功能测定与QCM-D分析相结合证明了具有前景的双重生物活性涂层表面。