Lapageria rosea is an emblematic species, endemic to the temperate rainforest of Chile. As a result of extensive plantation of commercial exotic pine and eucalypts, scattered individuals of L. rosea are now encountered mainly in fragmented forest areas. Therefore, L. rosea has been declared in danger of extinction. However, the success of national conservation programs depends, in part, on the availability of stored viable pollen grains for pollinating assays, in order to sustain the genetic diversity of L. rosea offspring. Here we describe a simple and low‐cost protocol for storing viable pollen grains for 1 year. The protocol involves oven‐drying freshly collected pollen grains at 37°C for 15 min, followed by storage at ‐13°C. The stored pollen has an average of 60–65% in vitro germination after 5 months, with a reduction to 50% by the end of 1 year, a value considered highly satisfactory to ensure the success of hand‐pollination trials.

中文翻译：

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储存Lapageria rosea（Philesiaceae）活花粉的新协议，Lapageria rosea是智利温带森林特有的濒危植物

Lapageria rosea是智利的温带雨林特有的象征物种。由于大量商业化的外来松树和桉树的种植，现在散布着玫瑰花的个体主要在零散的森林地区遇到。因此，已宣布玫瑰蔷薇有灭绝的危险。但是，国家保护计划的成功在一定程度上取决于为授粉试验保存的可行花粉粒的可用性，以维持玫瑰蔷薇的遗传多样性。后代。在这里，我们描述了一种简单且低成本的协议，用于将可行的花粉粒存储1年。该协议包括将新鲜收集的花粉粒在37°C下烘干15分钟，然后在-13°C下存储。储存的花粉在5个月后的平均体外发芽率为60-65％，到1年年底降低到50％，这一值被认为非常令人满意，可以确保手传授粉试验的成功。