Natural sex change in mature protogynous orange‐spotted grouper ( Epinephelus coioides ): gonadal restructuring, sex hormone shifts and gene profiles,Journal of Fish Biology

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Natural sex change in mature protogynous orange‐spotted grouper ( Epinephelus coioides ): gonadal restructuring, sex hormone shifts and gene profiles
Journal of Fish Biology ( IF 2 ) Pub Date : 2020-07-06 , DOI: 10.1111/jfb.14434
Cheng Peng _{1,

2} , Qing Wang _{2,

3} , Herong Shi ₄ , Jiaxing Chen ₂ , Shuisheng Li ₂ , Huihong Zhao ₃ , Haoran Lin ₂ , Jianchun Yang ₁ , Yong Zhang _{2,

4}

Affiliation

Sexual patterns of teleosts are extremely diverse and include both gonochorism and hermaphroditism. As a protogynous hermaphroditic fish, all orange-spotted groupers (Epinephelus coioides) develop directly into females, and some individuals change sex to become functional males later in life. In this study, we investigated gonadal restructuring, shifts in sex hormone levels, and gene profiles of cultured mature female groupers during the first (main) breeding season of 2019 in Huizhou, China (22°42'02.6"N, 114°32'10.1″E). Analysis of gonadal restructuring revealed that females with pre-vitellogenic ovaries went through vitellogenesis, spawning and regression and then returned to the pre-vitellogenic stage in the late breeding season, at which point some changed sex to become males via the intersex gonad stage. A significant decrease of serum 17β-estradiol (E2) level was observed during ovary regression but not during sex change, while serum 11-ketotestosterone (11-KT) concentrations increased significantly during sex change with the highest concentration in newly developed males. Consistent with serum hormone changes, a significant decrease in cyp19a1a expression was observed during ovary regression but not during sex change, while the expression of cyp11c1 and hsd11b2 increased significantly during sex change. Interestingly, hsd11b2 but not cyp11c1 was significantly upregulated from the pre-vitellogenic ovary stage to the early intersex gonad stage. These results suggest that a decrease in serum E2 concentration and downregulation of cyp19a1a expression are not necessary to trigger the female-to-male transformation, whereas increased 11-KT concentration and upregulation of hsd11b2 expression may be key events for initiation of sex change in the orange-spotted grouper.

更新日期：2020-07-06

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