Chitinase degrades chitin in the old epidermis or peritrophic matrix of insects, which ensures normal development and metamorphosis. In our previous work, we comprehensively studied the function of SfCht7 in Sogatella furcifera. However, the number and function of chitinase genes in S. furcifera remain unknown. Here, we identified 12 full-length chitinase transcripts from S. furcifera, which included nine chitinase (Cht), two imaginal disc growth factor (IDGF), and one endo-β-N-acetylglucosaminidase (ENGase) genes. Expression analysis results revealed that the expression levels of eight genes (SfCht3, SfCht5, SfCht6-1, SfCht6-2, SfCht7, SfCht8, SfCht10, and SfIDGF2) with similar transcript levels peaked prior to molting of each nymph and were highly expressed in the integument. Based on RNA interference (RNAi), description of the functions of each chitinase gene indicated that the silencing of SfCht5, SfCht10, and SfIDGF2 led to molting defects and lethality. RNAi inhibited the expressions of SfCht5, SfCht7, SfCht10, and SfIDGF2, which led to downregulated expressions of chitin synthase 1 (SfCHS1, SfCHS1a, and SfCHS1b) and four chitin deacetylase genes (SfCDA1, SfCDA2, SfCDA3, and SfCDA4), and caused a change in the expression level of two trehalase genes (TRE1 and TRE2). Furthermore, silencing of SfCht7 induced a significant decrease in the expression levels of three wing development-related genes (SfWG, SfDpp, and SfHh). In conclusion, SfCht5, SfCht7, SfCht10, and SfIDGF2 play vital roles in nymph–adult transition and are involved in the regulation of chitin metabolism, and SfCht7 is also involved in wing development; therefore, these genes are potential targets for control of S. furcifera.

中文翻译：

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参与Sogatella furcifera若虫-成虫转化的几丁质酶家族基因的表征和功能分析

几丁质酶降解昆虫旧表皮或围营养基质中的几丁质，保证昆虫的正常发育和变态。在我们之前的工作中，我们全面研究了SfCht7在Sogatella furcifera 中的功能。然而，S中几丁质酶基因的数量和功能。furcifera仍然未知。在这里，我们从S 中鉴定了 12 个全长几丁质酶转录本。furcifera，其中包括九种几丁质酶 ( Cht )、两种成虫盘生长因子 ( IDGF ) 和一种内切-β -N-乙酰氨基葡萄糖苷酶 ( ENGase)) 基因。表达分析结果表明，具有相似转录水平的8个基因（SfCht3、SfCht5、SfCht6-1、SfCht6-2、SfCht7、SfCht8、SfCht10和SfIDGF2）的表达水平在每只若虫蜕皮前达到峰值，并在外皮。基于 RNA 干扰 (RNAi)，对每个几丁质酶基因功能的描述表明，SfCht5、SfCht10和SfIDGF2的沉默导致蜕皮缺陷和致死率。RNAi抑制SfCht5、SfCht7的表达, SfCht10和SfIDGF2，导致几丁质合酶 1 ( SfCHS1、SfCHS1a和SfCHS1b ) 和四种几丁质脱乙酰酶基因 ( SfCDA1、SfCDA2、SfCDA3和SfCDA3和SfCDA4的表达水平的两个变化) 、基因（TRE1和TRE2）。此外，SfCht7 的沉默诱导三个翅膀发育相关基因（SfWG、SfDpp和SfHh）的表达水平显着降低。综上所述，SfCht5、SfCht7、SfCht10和SfIDGF2在若虫-成虫转化中起重要作用，参与几丁质代谢的调控，SfCht7也参与翅膀发育；因此，这些基因是控制S 的潜在目标。紫草。