With the availability of a new highly contiguous Bos taurus reference genome assembly (ARS‐UCD1.2), it is the opportune time to upgrade the bovine gene set by seeking input from researchers. Furthermore, advances in graphical genome annotation tools now make it possible for researchers to leverage sequence data generated with the latest technologies to collaboratively curate genes. For many years the Bovine Genome Database (BGD) has provided tools such as the Apollo genome annotation editor to support manual bovine gene curation. The goal of this paper is to explain the reasoning behind the decisions made in the manual gene curation process while providing examples using the existing BGD tools. We will describe the sources of gene annotation evidence provided at the BGD, including RNA‐seq and Iso‐Seq data. We will also explain how to interpret various data visualizations when curating gene models, and will demonstrate the value of manual gene annotation. The process described here can be applied to manual gene curation for other species with similar tools. With a better understanding of manual gene annotation, researchers will be encouraged to edit gene models and contribute to the enhancement of livestock gene sets.

中文翻译：

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使用牛基因组数据库中的在线工具手动注释新参考基因组中的基因。

随着新的高度连续的金牛座参考基因组组件（ARS‐UCD1.2）的出现，现在是时候通过寻求研究人员的意见来升级牛基因组。此外，图形化基因组注释工具的发展现在使研究人员可以利用最新技术生成的序列数据来共同管理基因。多年来，牛基因组数据库（BGD）提供了诸如Apollo之类的工具基因组注释编辑器支持手动牛基因管理。本文的目的是解释人工基因管理过程中做出的决定背后的原因，同时提供使用现有BGD工具的示例。我们将描述BGD提供的基因注释证据的来源，包括RNA-seq和Iso-Seq数据。我们还将说明在管理基因模型时如何解释各种数据可视化，并演示手动基因注释的价值。此处描述的过程可以通过相似的工具应用于其他物种的手动基因管理。有了对手动基因注释的更好理解，将鼓励研究人员编辑基因模型，并为增强牲畜基因集做出贡献。