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p300 promotes cell proliferation through suppressing Kaposi's sarcoma-associated herpesvirus (KSHV) reactivation in the infected B-lymphoma cells.
Virus Research ( IF 5 ) Pub Date : 2020-06-15 , DOI: 10.1016/j.virusres.2020.198066
Chuankai Sun 1 , Yizhen Guo 1 , Wei Zhou 2 , Chuan Xia 1 , Xiwen Xing 1 , Jun Chen 1 , Xin Li 1 , Hua Zhu 1 , Jie Lu 1
Affiliation  

Primary Effusion Lymphoma (PEL) is a B-cell lymphoma associated with Kaposi's sarcoma herpesvirus (KSHV) infection. However, the mechanism of oncogenesis of PEL is still unclear. Studies have shown that the cellular transcriptional coactivator p300 regulates the interaction between host and virus, which plays a vital role in viral replication. In this study, we investigated the role of p300 in BCBL1 cells during the KSHV life cycle. We found that p300 knockout resulted in an overall increase for the early lytic genes and changed the expression of genes associated with tumor development, proliferation, and the immune response in the KSHV infected B cells. However, knockout of p300 significantly inhibited the expression of the immediate-early gene RTA and the late lytic gene K8 after KSHV lytic activation. Additionally, the intracellular KSHV genome copy number and the virion production were reduced. These results demonstrated that p300 plays a crucial role in suppressing KSHV viral replication in BCBL1. Furthermore, we observed that the growth of BCBL1 was inhibited by knockout of p300, which confirmed our findings that p300 promotes cell proliferation. This study further provided evidence that p300 plays an important role in the pathogenesis of BCBL1, which might lead to the oncogenesis of PEL caused by KSHV infection.



中文翻译:

p300通过抑制感染的B淋巴瘤细胞中的卡波西氏肉瘤相关疱疹病毒(KSHV)活化来促进细胞增殖。

原发性淋巴瘤(PEL)是与卡波济氏肉瘤疱疹病毒(KSHV)感染相关的B细胞淋巴瘤。但是,PEL的致癌机制仍不清楚。研究表明,细胞转录共激活因子p300调节宿主与病毒之间的相互作用,这在病毒复制中起着至关重要的作用。在这项研究中,我们调查了KSHV生命周期中p300在BCBL1细胞中的作用。我们发现,p300基因敲除导致早期裂解基因的整体增加,并改变了与KSHV感染的B细胞中肿瘤发展,增殖和免疫反应相关的基因表达。但是,敲除p300会显着抑制KSHV裂解激活后立即早期基因RTA和晚期裂解基因K8的表达。另外,细胞内KSHV基因组拷贝数和病毒体产量减少。这些结果表明,p300在抑制BCBL1中的KSHV病毒复制中起着至关重要的作用。此外,我们观察到p300的敲除抑制了BCBL1的生长,这证实了我们的发现p300促进细胞增殖。这项研究进一步提供了证据,证明p300在BCBL1的发病机理中起重要作用,这可能导致由KSHV感染引起的PEL的癌变。

更新日期:2020-06-24
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