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A novel fluorescent probe with dual-sites for simultaneously monitoring metabolisms of cysteine in living cells and zebrafishes.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.4 ) Pub Date : 2020-06-15 , DOI: 10.1016/j.saa.2020.118602
Guoxing Yin 1 , Ting Yu 1 , Yabing Gan 1 , Li Zhou 2 , Meiling Liu 1 , Youyu Zhang 1 , Haitao Li 1 , Peng Yin 1 , Shouzhuo Yao 1
Affiliation  

Understanding cellular metabolism holds immense potential for developing new drugs that regulate metabolic pathways. Two gas signal molecules, SO2 and H2S, are the main metabolites from cysteine (Cys) via oxidation and desulfurization pathways, respectively. However, a few fluorescent probes for real-time monitor of the metabolic pathways of cysteine have been reported. To understand metabolic alterations of cysteine, we have rationally designed and prepared a dual-signal fluorescent probe HN, which could differentiate SO2 and H2S through two different fluorescence channels simultaneously, along with similar reaction kinetics and both “off-on” fluorescence responses. Probe HN exhibits the potential to monitor the metabolism pathways of cysteine, and the distinguishment of cancer cells from normal cells could be realized. This methodology will promote further understanding of the physiological and pathological roles of cysteine.



中文翻译:

具有双位点的新型荧光探针,可同时监测活细胞和斑马鱼中半胱氨酸的代谢。

了解细胞代谢具有开发调节代谢途径的新药的巨大潜力。两个气体信号分子SO 2和H 2 S分别是半胱氨酸(Cys)通过氧化和脱硫途径产生的主要代谢产物。然而,已经报道了一些用于实时监测半胱氨酸代谢途径的荧光探针。为了了解半胱氨酸的代谢变化,我们合理设计并制备了双信号荧光探针HN,该探针可通过两个不同的荧光通道同时区分SO 2和H 2 S,以及相似的反应动力学和“关闭”荧光回应。探针HN展示了监测半胱氨酸代谢途径的潜力,并且可以实现癌细胞与正常细胞的区分。这种方法将促进对半胱氨酸的生理和病理作用的进一步了解。

更新日期:2020-06-28
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