Abstract Mastitis causes significant economic losses in sheep flocks. Staphylococcus aureus is the most common microorganisms isolated in ovine clinical mastitis. To characterize S. aureus causing sheep mastitis in Algeria, 252 samples of clinical mastitis cases were collected and subjected to Staphylococcus aureus isolation, isolates were identified by PCR-RFLP and antimicrobial susceptibility was tested by the disk diffusion method. Spa type and antibiotic resistance genes were determined by PCR. In total, 69 S. aureus were isolated (69/252; 27.38%). Twenty-two isolates (22/69; 31.88%) were susceptible to all tested antibiotics whereas all isolates were methicillin-susceptible (MSSA). A high frequency of resistance to tetracycline (33.33%) and penicillin (17.39%) was detected, respectively. The tet(K), tet(L), bla(Z) and erm(B) genes were detected in resistant isolates. Multidrug resistance was detected only in one isolate. Fourteen different spa types were detected, predominantly t1773 (43/69; 62.32%), t967 (4/69; 5.79%) and t1534 (4/69; 5.79%). Three new spa types were found having the following repeats successions (04-31-17-24-25-17-17), (04-31-17-24-17-17) and (07-23-12-34-12-12-23-03-12-23). These results show that t1773 is the prevalent S. aureus spa type associated with ovine mastitis in Medea province and point out an emergence of antibiotic resistance of S. aureus isolates especially to tetracycline and penicillin. Further investigations will be needed to monitor the evolution of responsible epidemic strains and to improve methods for their control.

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阿尔及利亚美狄亚省临床绵羊乳腺炎金黄色葡萄球菌的分型及药敏分析

摘要 乳腺炎给羊群造成重大经济损失。金黄色葡萄球菌是在绵羊临床乳腺炎中分离到的最常见的微生物。为了表征在阿尔及利亚引起绵羊乳腺炎的金黄色葡萄球菌，收集了252个临床乳腺炎病例样本并对其进行了金黄色葡萄球菌分离，通过PCR-RFLP鉴定分离株，并通过圆片扩散法测试抗菌敏感性。通过PCR确定Spa类型和抗生素抗性基因。总共分离出 69 个金黄色葡萄球菌 (69/252; 27.38%)。22 个分离株 (22/69；31.88%) 对所有测试的抗生素敏感，而所有分离株都对甲氧西林敏感 (MSSA)。分别检测到对四环素 (33.33%) 和青霉素 (17.39%) 的高频率耐药性。tet(K), tet(L), 在耐药菌株中检测到 bla(Z) 和 erm(B) 基因。仅在一种分离株中检测到多药耐药性。检测到 14 种不同的温泉类型，主要是 t1773（43/69；62.32%）、t967（4/69；5.79%）和 t1534（4/69；5.79%）。发现了三种新的水疗类型，具有以下重复序列 (04-31-17-24-25-17-17)、(04-31-17-24-17-17) 和 (07-23-12-34- 12-12-23-03-12-23）。这些结果表明 t1773 是 Medea 省与绵羊乳腺炎相关的流行金黄色葡萄球菌 spa 类型，并指出金黄色葡萄球菌分离株出现了抗生素耐药性，尤其是对四环素和青霉素的耐药性。需要进一步调查以监测负责任流行毒株的演变并改进其控制方法。检测到 14 种不同的温泉类型，主要是 t1773（43/69；62.32%）、t967（4/69；5.79%）和 t1534（4/69；5.79%）。发现了三种新的水疗类型，具有以下重复序列 (04-31-17-24-25-17-17)、(04-31-17-24-17-17) 和 (07-23-12-34- 12-12-23-03-12-23）。这些结果表明 t1773 是 Medea 省与绵羊乳腺炎相关的流行金黄色葡萄球菌 spa 类型，并指出金黄色葡萄球菌分离株出现了抗生素耐药性，尤其是对四环素和青霉素的耐药性。需要进一步调查以监测负责任流行毒株的演变并改进其控制方法。检测到 14 种不同的温泉类型，主要是 t1773（43/69；62.32%）、t967（4/69；5.79%）和 t1534（4/69；5.79%）。发现了三种新的水疗类型，具有以下重复序列 (04-31-17-24-25-17-17)、(04-31-17-24-17-17) 和 (07-23-12-34- 12-12-23-03-12-23）。这些结果表明 t1773 是 Medea 省与绵羊乳腺炎相关的流行金黄色葡萄球菌 spa 类型，并指出金黄色葡萄球菌分离株出现了抗生素耐药性，尤其是对四环素和青霉素的耐药性。需要进一步调查以监测负责任流行毒株的演变并改进其控制方法。(04-31-17-24-17-17) 和 (07-23-12-34-12-12-23-03-12-23)。这些结果表明 t1773 是 Medea 省与绵羊乳腺炎相关的流行金黄色葡萄球菌 spa 类型，并指出金黄色葡萄球菌分离株出现了抗生素耐药性，尤其是对四环素和青霉素的耐药性。需要进一步调查以监测负责任流行毒株的演变并改进其控制方法。(04-31-17-24-17-17) 和 (07-23-12-34-12-12-23-03-12-23)。这些结果表明 t1773 是 Medea 省与绵羊乳腺炎相关的流行金黄色葡萄球菌 spa 类型，并指出金黄色葡萄球菌分离株出现了抗生素耐药性，尤其是对四环素和青霉素的耐药性。需要进一步调查以监测负责任流行毒株的演变并改进其控制方法。