The field assessment technique to evaluate the plants with a fungal phytopathogen for their tolerance to the disease is one of the crucial steps in dissecting their genetic control and in developing the resistant crop varieties. The objective behind this study was to develop and evaluate a field-based non-injury method of inoculation technique for Sclerotinia stem rot (SSR) in oilseed Brassica, caused by Sclerotinia sclerotiorum (Lib.) de Bary. The non-injury method of screening technique involves stem inoculation using a five days old mycelial mat on potato dextrose agar (PDA) plug placed on the top of sterile water-soaked cotton pad firmly wrapped over the internodal region with parafilm at the basal portion of the stem (15–20 cm above the ground) in the field. Inoculation without injury substantiates the natural means of infection in the field and the use of moist cotton pad keeps humidity for longer to initiate infection even in case of adverse climatic conditions. Disease development on the inoculated stem was measured by the length and width of the lesion. The symptom appears with water-soaked lesion formation and spreading deeper and wider on the stem in >90% of inoculated plants. During the experiment, about 800 Brassica germplasms including their wild relatives were screened and evaluated for three consecutive years using near-natural (non-injury) method of disease inoculation in the field. The Inoculation severity index (ISI) obtained during these years at Pusa, New Delhi were significantly similar and correlated with the natural infection measured in terms of disease severity index (DSI) on selected germplasm in the sick plot at ICAR-DRMR, Bharatpur. The significant correlations obtained among the used Brassica lines that were earlier not subjected for natural screening suggest the potential of this technique in evaluating the breeding material for SSR before confirmation with natural infection in the field.

评估具有真菌性植物病原体的植物对病害的耐受性的田间评估技术是剖析其遗传控制和开发抗性农作物品种的关键步骤之一。这项研究背后的目的是开发和油籽油菜，造成评估菌核病（SSR）接种技术的基于现场的非损伤的方法菌核病（库）德·巴里 非损伤性筛选技术包括在马铃薯葡萄糖琼脂（PDA）塞上使用五天龄的菌丝垫进行茎杆接种，该塞子置于无菌浸水的棉垫顶部，牢固地包裹在结间区域，并在基底的基础部分封膜。田间的茎（离地面15-20厘米）。接种无伤害即可证实田间自然的感染手段，即使在不利的气候条件下，使用潮湿的棉垫也可以长时间保持湿度，从而开始感染。通过病变的长度和宽度测量接种茎上的疾病发展。在超过90％的已接种植物中，症状表现为浸水的病斑形成并在茎上越来越深地扩散。在实验过程中，大约800个芸苔属在田间使用近自然（非伤害性）的疾病接种方法对包括其野生亲缘种质在内的种质进行了连续三年的筛选和评估。这些年来，在新德​​里的普萨获得的接种严重度指数（ISI）显着相似，并且与在巴拉特普尔的ICAR-DRMR病区的选定种质上的疾病种质的疾病严重度指数（DSI）衡量的自然感染相关。在使用过的未进行自然筛选的芸苔属品系之间获得的显着相关性表明，该技术在确定田间自然感染之前评估SSR育种材料的潜力。