The development of biomolecules adsorbent with superb efficiency and large processing throughput is of great importance for separation and reusing of enzymes in its free form in various vital industries. Herein, laccase enzyme adsorbent was prepared by carboxylation of nanofibrous nylon 6 membranes (Ny NFM) to form complex with copper metal ions [Ny-CCA-Cu(II)] with high selectivity for laccase adsorption with adsorption capacity 220 mg.g⁻¹ within 4 h. The prepared nylon NFM provides a promising candidate for separation and use of laccase enzyme in its free form for several times without significant change in its activity. Moreover, [Ny-CCA-Cu(II)] showed dynamic binding efficiency of 160 mg g⁻¹ which was achieved solely by a gravity filtration, as well as good reusability of [Ny-CCA-Cu(II)] at least for 5 cycles. The results were due to stable physical and chemical structures, highly tortuous open-porous structure of the nanofibrous membranes, and incorporated Cu⁺² complex in [Ny-CCA-Cu(II)]. This simple approach may introduce a way for the design and development of a cost-effective, environmentally friendly and selective adsorptive membranes to recover the valuable enzymes in industrial applications.

开发具有高效率和大处理量的生物分子吸附剂对于分离和再利用各种重要工业中的游离形式的酶至关重要。本文中，漆酶酶吸附剂是通过将纳米纤维尼龙6膜（Ny NFM）羧化以与铜金属离子[Ny-CCA-Cu（II）]形成络合物而制备的，具有高选择性，对漆酶的吸附能力为220 mg.g ^-1 4小时内 制备的尼龙NFM为分离和使用其游离形式的漆酶提供了有希望的候选方法，这种酶可以多次分离而不会改变其活性。此外，[Ny-CCA-Cu（II）]的动态结合效率为160 mg g ^-1仅通过重力过滤以及[Ny-CCA-Cu（II）]的良好重用性（至少5个循环）即可实现。结果是由于稳定的物理和化学结构，高度曲折的纳米纤维膜开孔结构以及在[Ny-CCA-Cu（II）]中掺入了Cu ⁺²络合物。这种简单的方法可能会引入一种设计和开发成本有效，环保且选择性吸附膜的方法，以在工业应用中回收有价值的酶。