One of the most severe diseases affecting soybean is the Asian Soybean Rust (ASR) caused by Phakopsora pachyrhizi H. Sydow & P. Sydow. The oxidative damage in cellular constituents and the disruption in the photosynthetic apparatus are the major negative effects of ASR on soybean plants. Considering the importance of nickel (Ni) on plant metabolism, this study evaluated the effect of foliar Ni-supply on ASR control, reactive oxygen species (ROS) accumulation, activities of antioxidant enzymes, parameters related to photosynthesis, and sugars levels on soybean plants. ASR severity decreased by 34% due to Ni supply. Infected and Ni-supplied (+ Ni) plants showed lower malondialdehyde level and superoxide (O²⁻) and hydrogen peroxide (H₂O₂) accumulation in contrast to infected leaf tissues of non-Ni-supplied (− Ni) plants. The antioxidant enzymes activities were inefficient to avoid the high ROS accumulation for − Ni inoculated plants. The photosynthetic pigments, maximum photochemical efficiency of photosystem II (PSII), effective yield of PSII, electron transport rate, rate of net carbon assimilation, stomatal conductance to water vapor, and transpiration rate values were higher and the yield for other non-regulated losses and internal CO₂ concentration values were lower for + Ni inoculated plants compared to − Ni inoculated plants. High ROS production and the greater damage to the photosynthetic apparatus caused by P. pachyrhizi infection on − Ni plants affected the synthesis of the sugars. The infection also decreased the plant’s energetic reserves faster in − Ni plants compared to + Ni plants. In conclusion, the cellular oxidative damage and the impairment on the photosynthetic apparatus of soybean plants caused by P. pachyrhizi infection were alleviated by supplying Ni foliarly.

影响大豆的最严重疾病之一是由Phakopsora pachyrhizi H. Sydow和P. Sydow引起的亚洲大豆锈病（ASR）。细胞成分中的氧化损伤和光合装置的破坏是ASR对大豆植物的主要负面影响。考虑到镍（Ni）对植物代谢的重要性，本研究评估了叶面镍供应对大豆植物ASR控制，活性氧（ROS）积累，抗氧化酶活性，与光合作用相关的参数以及糖水平的影响。由于镍的供应，ASR严重性降低了34％。受感染和由Ni提供的（+ Ni）植物显示出较低的丙二醛水平和超氧化物（O ²⁻）和过氧化氢（H ₂ O ₂）与非Ni供应的（-Ni）植物的感染叶子组织形成对比。对于接种-Ni的植物，抗氧化酶活性无法有效地避免较高的ROS积累。光合色素，光系统II（PSII）的最大光化学效率，PSII的有效产率，电子传输速率，净碳同化率，气孔对水蒸气的电导率以及蒸腾速率值较高，而其他非调节损失的产率较高和+ Ni接种植物相比，+ Ni接种植物的内部CO ₂浓度值更低。Pachyrhizi引起的高ROS产生和对光合装置的更大破坏镍植物上的感染影响了糖的合成。与+ Ni植株相比，感染还使− Ni植株更快地降低了植物的能量储备。综上所述，叶面供应镍减轻了P. pyryrhizi感染引起的大豆细胞氧化损伤和光合装置损伤。