Abstract The stable fly, Stomoxys calcitrans (Linnaeus 1758), is a hematophagous fly responsible for causing loss of performance in horses, causing losses in cattle productivity, and impacting the animals' health through the spread of pathogenic microorganisms. The objective of this work was to investigate the insecticidal activity of essential oil obtained from Melaleuca alternifolia (Cheel), presenting high 1,8-cineole content, against S. calcitrans adults. Insecticidal activity was determined using surface application methods and exposure to oil impregnated paper. It was observed that treatments at 25 and 50 µg/cm2 (P < 0.05) present fumigant activity through exposure to the impregnated paper, and in the first 15 min of exposure, the mortality rates obtained for these treatments were, respectively (96.6 ± 3.3% and 100%), equivalent to the positive control. Using the superficial application method, the only treatment concentration presenting adulticidal action was 5% (w/v) (P < 0.05). Respective toxicities LC50 (%, w/v) and LC80 for the impregnated paper method were 1.06 ± 0.02 and 1.47 ± 0.17; for the superficial application method, they were 3.82 ± 0.65 and 5.53 ± 0.74. As demonstrated, M. alternifolia essential oil presents adulticidal potential against S. calcitrans.

中文翻译：

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具有高 1,8-桉树脑含量的互叶白千层（桃金娘属：桃金娘科）精油对稳定苍蝇（双翅目：麝香科）的杀成虫活性

摘要 稳定的苍蝇 Stomoxys calcitrans (Linnaeus 1758) 是一种食血苍蝇，它会导致马的性能下降、牛的生产力下降，并通过病原微生物的传播影响动物的健康。这项工作的目的是研究从互叶白千层 (Cheel) 中提取的精油对 S. calcitrans 成虫的杀虫活性，该精油具有高 1,8-桉树脑含量。使用表面施用方法和暴露于油浸纸来测定杀虫活性。据观察，25 和 50 µg/cm2 (P < 0.05) 的处理通过暴露于浸渍纸表现出熏蒸活性，并且在暴露的前 15 分钟，这些处理获得的死亡率分别为 (96.6 ± 3.3 % 和 100%), 相当于阳性对照。使用表面施用方法，表现出杀成虫作用的唯一处理浓度为 5% (w/v) (P < 0.05)。浸渍纸法的毒性 LC50 (%, w/v) 和 LC80 分别为 1.06 ± 0.02 和 1.47 ± 0.17；表涂法分别为3.82±0.65和5.53±0.74。正如所证明的，互叶梅精油对 S. calcitrans 具有杀成虫的潜力。