Abstract

This study is aimed to investigate the influence of three pluronics (F127, F87, and P85) on the structure, stability, and cytotoxicity of liposomes (Ls). Pluronics-modified liposomes (pluronic-Ls) were prepared by integrating the film evaporation method and dynamic high pressure microfluidization. The average particle size of liposomes decreased significantly after modification with pluronics. Fourier transform infrared spectroscopy and differential scanning calorimetry analysis revealed that pluronics were successfully incorporated into liposomes. The membrane stability of liposomes was improved by pluronics modification, and increased in the following order: Ls < F87-Ls < F127-Ls < P85-Ls. In vitro cytotoxicity studies indicated that F127-Ls and F87-Ls possess good biocompatibility, and P85-Ls exhibited relatively low inherent toxicity in the phospholipid concentrations ranging from 100 to 400 mg/L. These results demonstrated that pluronics F127 and F87 are the optimal agents for liposomes modification and can potentially be used as a drug delivery platform.

摘要

本研究旨在研究三种普朗尼克（F127、F87 和 P85）对脂质体 (Ls) 的结构、稳定性和细胞毒性的影响。将薄膜蒸发法和动态高压微流化相结合，制备了Pluronics修饰的脂质体（pluronic-Ls）。用pluronics修饰后脂质体的平均粒径显着降低。傅里叶变换红外光谱和差示扫描量热分析表明，pluronics 已成功掺入脂质体中。脂质体的膜稳定性通过pluronics修饰得到改善，并按以下顺序增加：Ls < F87-Ls < F127-Ls < P85-Ls。体外细胞毒性研究表明，F127-Ls 和 F87-Ls 具有良好的生物相容性，而 P85-Ls 在 100 至 400 mg/L 的磷脂浓度范围内表现出相对较低的固有毒性。这些结果表明，pluronics F127 和 F87 是脂质体修饰的最佳试剂，有可能用作药物递送平台。