A Fe‐porphyrin covalent organic framework (Fe‐PorCOF) was prepared through a postmodification strategy and characterized using different techniques. Fe‐PorCOF exhibits an inherent peroxidase/oxidase mimetic catalytic activity and sharply accelerates chemiluminescence (CL) reactions between luminol and hydrogen peroxide (H₂O₂) or dissolved oxygen (O₂) under alkaline conditions. The catalytic role was attributed to a significant increase in production of reactive oxygen species. Using the imminent peroxidase mimetic catalytic activity of Fe‐PorCOF, a new CL method was developed for determination of H₂O₂ over a linear range from 0.01 to 10.0 μmol·L⁻¹ and with a limit of detection of 5.3 nmol·L⁻¹. The combination of the peroxidase mimetic catalytic activity of Fe‐PorCOF with the catalytic activity of glucose oxidase on glucose oxidation presents a sensitive CL method for glucose assay. The linear range and the detection limit for glucose were 0.05–8.0 μmol·L⁻¹ and 4.0 nmol·L⁻¹, respectively. The practicability of this method was assessed by determination of glucose in human sera. As a peroxidase/oxidase mimetic, Fe‐PorCOF is easy to prepare and exhibits good catalytic efficiency in the luminol reaction. We believe that this strategy will promote the development of a CL field with functional COFs as a catalyst.

中文翻译：

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一种新型的过氧化物酶/氧化酶模拟铁卟啉共价有机框架增强了鲁米诺化学发光反应及其在葡萄糖传感中的应用。

通过后修饰策略制备了Fe-卟啉共价有机骨架（Fe-PorCOF），并使用不同的技术对其进行了表征。Fe-PorCOF表现出固有的过氧化物酶/氧化酶模拟催化活性，并在碱性条件下急剧加速鲁米诺与过氧化氢（H ₂ O ₂）或溶解氧（O ₂）之间的化学发光（CL）反应。催化作用归因于活性氧物质的产生的显着增加。利用Fe‐PorCOF即将出现的过氧化物酶模拟催化活性，开发了一种新的CL方法，用于测定0.01至10.0μmol·L ^-1的线性范围内的H ₂ O ₂。检测极限为5.3nmol·L ^-1。Fe‐PorCOF的过氧化物酶模拟催化活性与葡萄糖氧化酶对葡萄糖氧化的催化活性相结合，为葡萄糖测定提供了一种灵敏的CL方法。葡萄糖的线性范围和检出限分别为0.05–8.0μmol·L ^-1和4.0nmol·L ^{- 1}。该方法的实用性通过测定人血清中的葡萄糖来评估。作为过氧化物酶/氧化酶的模拟物，Fe-PorCOF易于制备，并且在鲁米诺反应中显示出良好的催化效率。我们认为，该策略将促进功能性COF作为催化剂的CL领域的发展。