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Accumulation of Polyphenolic Compounds and Osmolytes under Dehydration Stress and Their Implication in Redox Regulation in Four Indigenous Aromatic Rice Cultivars
Rice Science ( IF 4.8 ) Pub Date : 2020-06-13 , DOI: 10.1016/j.rsci.2020.05.008
Nivedita Dey , Soumen Bhattacharjee

Present work was undertaken to screen some drought tolerant indigenous aromatic rice cultivars (IARCs), commonly cultivated in West Bengal, India, based on their capacity to produce osmolytes, redox-sensitive phenolic acids and flavonoids, as contrivances for redox-regulation under drought stress. Polyethylene glycol induced post imbibitional dehydration stress mediated changes in redox regulatory properties of the germinating seeds of the four IARCs (Jamainadu, Tulaipanji, Sitabhog, Badshabhog), which were assessed in terms of changes in prooxidant accumulation (in-situ localization of reactive oxygen species (ROS) by confocal microscopy, DCFDA (2′,7′-dichlorofluorescin diacetate) oxidation, O2 and H2O2 accumulation), cumulative antioxidative defense (radical scavenging property and total thiol content), ROS scavenging phenolic acids (gallic acid, protocatechuic acid, gentisic acid, para-hydroxy benzoic acid, chlorogenic acid, caffeic acid, syringic acid, salicylic acid, sinapic acid and p-coumaric acid) and flavonoids (catechin, naringin, rutin, quercetin, kaempferol, myricetin and apigenin). The capability of germinating seeds to accumulate osmolytes (like glycinebetaine, proline, soluble carbohydrates and K+ ion) and polyphenolic compounds was also correlated with their corresponding redox status and redox biomarkers (conjugated diene, hydroperoxide, thiobarbituric acid reactive substances and free carbonyl content) produced under the same conditions. The results in general showed that accumulation of osmolytes along with the redox-sensitive phenolics and flavonoids conferred the ability to maintain the redox homeostasis under drought stress for the tolerant IARCs (Badshabhog and Tulaipanji).



中文翻译:

干旱胁迫下4个土著水稻品种中多酚类化合物和渗透溶质的积累及其在氧化还原调控中的意义

目前的工作是根据其生产渗透液,氧化还原敏感酚酸和类黄酮的能力,筛选一些通常在印度西孟加拉邦种植的耐旱本地芳香水稻品种(IARC),作为干旱胁迫下氧化还原调节的贡献。聚乙二醇诱导的无限制的脱水胁迫介导了四个IARC(Jamainadu,Tulaipanji,Sitabhog,Badshabhog)发芽种子的氧化还原调节特性的变化,这些变化是根据促氧化剂积累的变化(活性氧的原位定位) (ROS)通过共聚焦显微镜,DCFDA(2',7'- dichlorofluorescin二乙酸酯)氧化,O- 2 -和H 2 ö 2累积),累积抗氧化防御(自由基清除性能和总硫醇含量),ROS清除酚酸(没食子酸,原儿茶酸,龙胆酸,对羟基苯甲酸,绿原酸,咖啡酸,丁香酸,水杨酸,芥子酸和对香豆酸)和类黄酮(儿茶素,柚皮苷,芦丁,槲皮素,山奈酚,杨梅素和芹菜素)。发芽种子积累渗透压的能力(如甘氨酸甜菜碱,脯氨酸,可溶性碳水化合物和钾离子离子和多酚化合物也与它们在相同条件下产生的氧化还原状态和氧化还原生物标记(共轭二烯,氢过氧化物,硫代巴比妥酸反应性物质和游离羰基含量)相关。总的来说,结果表明渗透剂与氧化还原敏感性酚类和类黄酮的积累赋予了耐旱的IARC(Badshabhog和Tulaipanji)在干旱胁迫下维持氧化还原稳态的能力。

更新日期:2020-06-13
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