The aim of the work was studying the effects of photobiomodulation of a red spectrum in doses of less than 1 J/cm² in combination with gamma-irradiation to Hela Kyoto cells. Tumor cells were irradiated with 640 nm LED at different energy densities before and after to gamma-irradiation. Cells viability was determined 24 h after exposure for each gamma-irradiation dose and each PBM mode. There was a statistically significant decrease in a number of viable tumor cells for the samples that were exposed to low-intensity red light prior to gamma-irradiation and a statistically significant increase in a number of viable tumor cells for the samples that were exposed to low-intensity red light after gamma-irradiation. An increase in the number of viable tumor cells exposed to PBM after gamma irradiation correlates with a decrease in the number of cells with a depolarized mitochondrial membrane. The results of a current study need to take into consideration at further studies of PBM effects on tumor cells in vitro as far as clinical studies and clinical application of PBM during radiation therapy.

这项工作的目的是研究剂量小于1 J / cm ²的红色光谱的光生物调节作用。结合伽马射线照射到Hela Kyoto细胞。γ射线照射前后，以不同的能量密度用640 nm LED照射肿瘤细胞。在暴露后24小时，对于每种γ-辐射剂量和每种PBM模式，确定细胞活力。在伽玛射线辐照之前暴露于低强度红光的样品中，存活的肿瘤细胞数量统计上显着减少，对于暴露于低剂量的样品，存活肿瘤细胞的数量上统计学上显着增加-伽马射线辐照后发出的红色强光。γ射线照射后，暴露于PBM的存活肿瘤细胞数量增加与线粒体去极化的细胞数量减少有关。