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Serum metabolomics identifies metabolite panels that differentiate lame dairy cows from healthy ones.
Metabolomics ( IF 3.6 ) Pub Date : 2020-06-13 , DOI: 10.1007/s11306-020-01693-z
Guanshi Zhang 1, 2 , Grzegorz Zwierzchowski 1, 3 , Rupasri Mandal 4 , David S Wishart 4 , Burim N Ametaj 1
Affiliation  

Introduction

Although much is known about lameness application of metabolomics technologies to better understanding its etiology and pathogenesis is of utmost interest.

Objectives

The objective of this study was to investigate serum metabolite alterations in pre-lame, lame and post-lame dairy cows in order to identify potential screening serum metabolite biomarkers for lameness and better understand its pathobiology.

Methods

A combination of direct injection and tandem mass spectrometry (DI–MS/MS) with a reverse-phase liquid chromatography and tandem mass spectrometry (LC–MS/MS) analysis was performed in the serum of six cases of lameness and 20 healthy control cows (CON) at − 8 and − 4 weeks prepartum, at lameness diagnosis week, and at + 4 and + 8 weeks postpartum.

Results

Data indicated that pre-lame, lame, and post-lame cows experienced altered concentrations of multiple metabolites. It is interesting to note that throughout the 16-weeks of the study, 7 serum metabolites [e.g., diacyl-phosphatidylcholine (PC aa) C30:0, phosphatidylcholine acyl-alkyl (PC ae) C40:2, sphingomyelin (SM) (OH) C14:1, SM C18:0, isoleucine (Ile), leucine (Leu), and lysine (Lys)] differentiated CON cows from the lame ones. Furthermore, 4 metabolic pathways (i.e., Lys degradation, biotin metabolism, tryptophan (Trp) metabolism, and valine [(Val)-Leu-Ile degradation) were altered in cows with lameness during the onset and progression of the disease.

Conclusion

Multiple metabolite and pathway alterations were identified in the serum of pre-lame, lame, and post-lame cows that through light into the pathobiology of the disease and that can be used as potential biomarker sets that can predict the risk of lameness in dairy cows.



中文翻译:

血清代谢组学可以鉴定出将la脚奶牛与健康牛区分开的代谢产物。

介绍

尽管对于代谢组学技术的la行应用了解很多,以更好地了解其病因和发病机制是最重要的。

目标

这项研究的目的是调查-子,la子和-子后奶牛的血清代谢物变化,以鉴定潜在的筛查serum子的血清代谢物生物标志物,并更好地了解其病理生物学。

方法

在六例cases足病和20头健康对照母牛的血清中进行了直接注射和串联质谱分析(DI–MS / MS)与反相液相色谱和串联质谱分析(LC–MS / MS)的组合(CON)在产前− 8和− 4周,la行诊断周以及产后+ 4和+ 8周。

结果

数据表明,-足、,足和post足后母牛的多种代谢产物浓度发生了变化。有趣的是,在整个研究的16周中,有7种血清代谢物[例如,二酰基磷脂酰胆碱(PC aa)C30:0,磷脂酰胆碱酰基烷基(PC ae)C40:2,鞘磷脂(SM)(OH )C14:1,SM C18:0,异亮氨酸(Ile),亮氨酸(Leu)和赖氨酸(Lys)]将CON母牛与la腿母牛区分开来。此外,在这种疾病的发作和发展过程中,有la腿病的母牛改变了4种代谢途径(即Lys降解,生物素代谢,色氨酸(Trp)代谢和缬氨酸[(Val)-Leu-Ile降解)]。

结论

在-行、,行和and行后的母牛的血清中鉴定出多种代谢物和途径改变,这些改变通过光进入疾病的病理生物学状态,可以用作潜在的生物标记物,可以预测奶牛cow行的风险。

更新日期:2020-06-13
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