The aim of the work was to use an effluent with high chemical oxygen demand (COD) from small brewery as substrate for the production of Chlorella vulgaris biomass. A non-axenic strain from a Patagonian river was employed, and the effects of COD and pH value on the microalga growth were studied through a central composite design. A medium with COD 18300 mg O₂ mL⁻¹ and initial pH: 6.5 optimized microalga growth. With the optimal condition, C. vulgaris adapted rapidly to medium and stationary phase was attained at 75 h. Moreover, lack of illumination did not affect μ_max, neither final biomass concentration, while supplementation with BG11 enhanced the biomass productivity at 0.47 ± 0.07 g L⁻¹ h⁻¹, and pigment contents at least four times respect to the heterotrophic mode. For the first time, a brewery wastewater with high COD could be successfully used as substrate for C. vulgaris production, without presenting inhibitions, which represents a significant advance contributing toward more sustainable promising perspectives.

该工作的目的是使用来自小型啤酒厂的具有高化学需氧量（COD）的废水作为生产小球藻生物质的基质。使用来自巴塔哥尼亚河的非树莓菌株，并通过中央复合设计研究了COD和pH值对微藻生长的影响。COD为18300 mg O ₂ mL ^-1且初始pH为6.5的培养基可优化微藻生长。在最佳条件下，寻常型梭状芽胞杆菌在75 h即可迅速适应中等和固定相。此外，缺少照明的不影响μ_最大，既不最终生物质浓度，而补充BG11在0.47±0.07克L-增强了生物质生产力^-1  h ^-1，并且颜料含量是异养模式的至少四倍。具有高化学需氧量的啤酒废水首次可以成功地用作生产小球藻的底物，而又没有任何抑制作用，这代表了朝着更可持续的前景发展的方向发展的重大进步。