Development of miniaturized devices has yielded many advantages, such as automation, portability and robustness. Out of several detection schemes, electrochemical sensing is the preferred choice due to its selectivity, accuracy and repeatability. In the present work, a miniaturized three-electrode electrochemical device has been fabricated on a glass substrate using inkjet printing of carbon conductive inks for sensing of xanthine (X), hypoxanthine (HX) and uric acid (UA). Hereby, the electroactive graphitized mesoporous carbon modified on conductive carbon paste acted as working electrode. The electrochemical behaviors of these purines were tested using cyclic voltammetry and squarewave voltammetry (SWV). The morphology and structural properties were characterized using scanning electron microscopy. Under the optimized conditions, the linear ranges for X, HX and UA were 2–12, 10–20 and 1–7 µM, respectively. The limit of detections for all these purines are 3.33, 9.99 and 0.01 µM for X, HX and UA, respectively. The developed platform was also utilized for interference with other co-existing bio-chemicals manifesting negligible interference. Further, the platform was successfully tested with human serum samples for X, HX and UA detection. Conclusively, the modified electrodes showed excellent reproducibility and specificity applicable to real samples.

小型设备的开发产生了许多优势，例如自动化，便携性和鲁棒性。在多种检测方案中，由于其选择性，准确性和可重复性，电化学传感是首选。在当前的工作中，已经使用碳导电油墨的喷墨印刷在玻璃基板上制造了小型化的三电极电化学装置，用于感测黄嘌呤（X），次黄嘌呤（HX）和尿酸（UA）。因此，在导电碳浆上改性的电活性石墨化介孔碳用作工作电极。这些嘌呤的电化学行为使用循环伏安法和方波伏安法（SWV）进行了测试。使用扫描电子显微镜表征形态和结构性质。在优化条件下 X，HX和UA的线性范围分别为2-12、10-20和1-7 µM。所有这些嘌呤的X，HX和UA的检出限分别为3.33、9.99和0.01 µM。所开发的平台还用于与其他共存的生物化学物质的干扰，这些干扰表现为微不足道的干扰。此外，该平台已成功使用人血清样品进行了X，HX和UA检测。最终，改性电极显示出可应用于真实样品的出色重现性和特异性。该平台已成功通过人血清样品进行X，HX和UA检测。最终，改性电极显示出可应用于真实样品的出色重现性和特异性。该平台已成功通过人血清样品进行X，HX和UA检测。最终，改性电极显示出可应用于真实样品的出色重现性和特异性。