Light sheet fluorescence microscopy has become a basic tool in biology and medical research with its fast imaging speed, low phototoxicity and high spatiotemporal resolution. Here, we report a novel method to generate multiplane parallel light sheets with a micro structure named isosceles triangular array. The thickness of light sheet in each plane can approach 3.12 μm along with the working distance of 152.20 μm directly generated with the isosceles triangular array. We experimentally recorded the multiplane parallel light sheets illumination, which is consistent with the corresponding simulation by using ray-tracing method. To evaluate the imaging quality, we compared the light field generated with and without projected phase mask onto the spatial light modulator by using polystyrene fluorescent microspheres nanoparticles and transgenic zebrafish. This study provides a potential method for the building of volumetric imaging light sheet fluorescence microscopy with high resolution and low phototoxicity.

中文翻译：

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更薄和更长的工作距离光片照明和显微成像

光片荧光显微镜以其成像速度快、光毒性低和时空分辨率高等优点成为生物学和医学研究的基本工具。在这里，我们报告了一种新方法来生成具有名为等腰三角形阵列的微结构的多平面平行光片。等腰三角形阵列直接产生152.20μm的工作距离，每个平面的光片厚度可达3.12μm。我们实验记录了多平面平行光片照明，这与使用光线追踪方法的相应模拟一致。为了评估成像质量，我们比较了使用聚苯乙烯荧光微球纳米粒子和转基因斑马鱼在空间光调制器上使用和不使用投影相位掩模生成的光场。该研究为构建具有高分辨率和低光毒性的体积成像光片荧光显微镜提供了一种潜在的方法。