Abstract Reducing the Cd content in crop plants is an important process for ensuring the safety of food made from the crop. In this study, the CMV△2b vector was used to express the PvSR2 gene to reduce the Cd content of Nicotiana benthamiana plants. The infectious clone plasmid pCB301-CMVFny209△2b-PvSR2 was successfully constructed. N. benthamiana plants at six leaf stages were inoculated with plasmid-Agrobacterium (GV3101) containing pCB301-CMVFny209△2b-PvSR2, pCB301-CMVFny109 and pCB301-CMVFny309 by agroinfiltration. After the virus had been detected in the non-inoculated leaves of the plant inoculated with plasmid-agrobacterium corresponding to each treatment, the plant of each treatments in the experiment were inoculated with sap of plant leaves containing the virus. After 8 days, the plants were treated with Cd++ solution. The Cd content of the plant samples was analyzed via inductively coupled plasma atomic emission spectrometry. The results show that the plants expressing the PvSR2 gene had greatly reduced Cd++ content than the control and grow normally. These results indicated that using the attenuated recombinant virus CMV△2b containing the PvSR2 gene significantly enhanced the resistance of the plant to Cd++. This is a new approach to protect tobacco plants from Cd contamination by using the attenuated recombinant virus CMV△2b containing the PvSR2 gene.

中文翻译：

---

使用含有 PvSR2 基因的减毒重组病毒 CMV△2b 保护烟草植物免受镉污染的新方法

摘要 降低农作物的镉含量是保证农作物食品安全的重要过程。本研究利用CMV△2b载体表达PvSR2基因以降低本氏烟草植株的Cd含量。感染性克隆质粒pCB301-CMVFny209△2b-PvSR2构建成功。通过农杆菌浸润，用含有 pCB301-CMVFny209△2b-PvSR2、pCB301-CMVFny109 和 pCB301-CMVFny309 的质粒-农杆菌 (GV3101) 接种本氏烟草植物的六个叶期。在接种了与各处理对应的质粒农杆菌的植物的未接种叶片中检测到病毒后，将实验中各处理的植物用含有该病毒的植物叶片的汁液接种。8天后，用Cd++溶液处理植物。通过电感耦合等离子体原子发射光谱法分析植物样品的镉含量。结果表明，表达PvSR2基因的植株与对照相比，Cd++含量大大降低，生长正常。这些结果表明，使用含有PvSR2基因的减毒重组病毒CMV△2b显着增强了植物对Cd++的抗性。这是一种利用含有PvSR2基因的减毒重组病毒CMV△2b保护烟草植株免受Cd污染的新方法。这些结果表明，使用含有PvSR2基因的减毒重组病毒CMV△2b显着增强了植物对Cd++的抗性。这是一种利用含有PvSR2基因的减毒重组病毒CMV△2b保护烟草植株免受Cd污染的新方法。这些结果表明，使用含有PvSR2基因的减毒重组病毒CMV△2b显着增强了植物对Cd++的抗性。这是一种利用含有PvSR2基因的减毒重组病毒CMV△2b保护烟草植株免受Cd污染的新方法。