The aim of this study is to develop a method for the synthesis of inulin butyrate ester (IBE) as a short chain fatty acid (SCFA) delivery carrier. Inulin is modified by butyric anhydride and the structure is characterized by Fourier Transform Infrared and ¹H NMR. On this basis, the synthetic conditions are optimized with response surface methodology (RSM) to investigate the effects of three parameters (the ratio of anhydride to inulin, inulin concentration, and reaction temperature) on the degree of substitution (DS). Under the optimal conditions, the actual experimental DS is 2.59 ± 0.14. The antioxidant activities of inulin and IBE are determined by superoxide anion radical and hydroxyl radical scavenging ability. The results show that the butyrylation inulin increases antioxidant activities and indicate that IBE could be used as a potential antioxidant. In a 36 h in vitro fermentation experiment, IBE is shown to increase the total SCFA content and the butyrate ratio, which illustrates that IBE could be used as SCFA carrier for delivering high concentration of SCFA to the colon, and thus influencing intestinal health.

中文翻译：

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菊粉丁酸酯的合成，表征，抗氧化活性及体外对SCFA生产的影响

这项研究的目的是开发一种合成菊糖丁酸酯（IBE）作为短链脂肪酸（SCFA）递送载体的方法。菊粉被丁酸酐改性，其结构的特征在于傅立叶变换红外光谱和¹1 H NMR。在此基础上，利用响应面法（RSM）优化了合成条件，以研究三个参数（酸酐与菊粉的比例，菊粉浓度和反应温度）对取代度（DS）的影响。在最佳条件下，实际实验DS为2.59±0.14。菊粉和IBE的抗氧化活性取决于超氧阴离子自由基和羟自由基的清除能力。结果表明，丁酰化菊粉增加了抗氧化剂的活性，并表明IBE可用作潜在的抗氧化剂。在36小时的体外发酵实验中，IBE可以提高总SCFA含量和丁酸酯比，这说明IBE可用作SCFA载体，可将高浓度的SCFA输送至结肠，